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Natural Insoluble Materials as Potential Support for Enzyme Immobilization

Enzyme is the heart of biotechnological processes as it permits 'green' synthesis at mild working conditions and consumes less energy. However, enzyme activities are limited by deleterious effects and extreme conditions. The need to produce or design enzyme with high activities and stab...

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Main Author: S.S. Othman
Format: Conference Paper
Language:English
Published: 2013
Subjects:
Immobilization > Natural materials > Support > Activities > Stabilities
Online Access:http://ddms.usim.edu.my/handle/123456789/6129
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spelling my.usim-61292015-07-03T00:50:22Z Natural Insoluble Materials as Potential Support for Enzyme Immobilization S.S. Othman Immobilization--Natural materials--Support--Activities--Stabilities Enzyme is the heart of biotechnological processes as it permits 'green' synthesis at mild working conditions and consumes less energy. However, enzyme activities are limited by deleterious effects and extreme conditions. The need to produce or design enzyme with high activities and stabilities for the green synthesis is therefore, a new challenge for scientists. In this study, the effects of natural insoluble materials, mainly plagioclase feldspar, mica and fibres of kenalf, were extensively explored for their use as support for enzyme immobilization. The use of these materials is advantageous as they can be found in abundant, possess good physico-chemical properties and are least expensive. Following a simple and straightforward, physical adsorption technique of immobilization, the natural materials exhibited high enzyme loading of 60-90% and enhanced enzyme activity by 4-8 folds, when tested in the syntheses of fine chemicals. Enzyme activities and stabilities were retained even after long exposure (10 days) to extremely polar organic solvents (log P = -0.3), extreme storage conditions and high temperature (70 °C). The immobilized enzymes also showed promising recyclability and kinetic profile as compared to native enzyme. The enzyme preparations produced has displayed promising action as biocatalysts for industrial applications. 2013-01-04T08:16:05Z 2013-01-04T08:16:05Z 2013-01-04 Conference Paper http://ddms.usim.edu.my/handle/123456789/6129 en
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Immobilization--Natural materials--Support--Activities--Stabilities
spellingShingle Immobilization--Natural materials--Support--Activities--Stabilities
S.S. Othman
Natural Insoluble Materials as Potential Support for Enzyme Immobilization
description Enzyme is the heart of biotechnological processes as it permits 'green' synthesis at mild working conditions and consumes less energy. However, enzyme activities are limited by deleterious effects and extreme conditions. The need to produce or design enzyme with high activities and stabilities for the green synthesis is therefore, a new challenge for scientists. In this study, the effects of natural insoluble materials, mainly plagioclase feldspar, mica and fibres of kenalf, were extensively explored for their use as support for enzyme immobilization. The use of these materials is advantageous as they can be found in abundant, possess good physico-chemical properties and are least expensive. Following a simple and straightforward, physical adsorption technique of immobilization, the natural materials exhibited high enzyme loading of 60-90% and enhanced enzyme activity by 4-8 folds, when tested in the syntheses of fine chemicals. Enzyme activities and stabilities were retained even after long exposure (10 days) to extremely polar organic solvents (log P = -0.3), extreme storage conditions and high temperature (70 °C). The immobilized enzymes also showed promising recyclability and kinetic profile as compared to native enzyme. The enzyme preparations produced has displayed promising action as biocatalysts for industrial applications.
format Conference Paper
author S.S. Othman
author_facet S.S. Othman
author_sort S.S. Othman
title Natural Insoluble Materials as Potential Support for Enzyme Immobilization
title_short Natural Insoluble Materials as Potential Support for Enzyme Immobilization
title_full Natural Insoluble Materials as Potential Support for Enzyme Immobilization
title_fullStr Natural Insoluble Materials as Potential Support for Enzyme Immobilization
title_full_unstemmed Natural Insoluble Materials as Potential Support for Enzyme Immobilization
title_sort natural insoluble materials as potential support for enzyme immobilization
publishDate 2013
url http://ddms.usim.edu.my/handle/123456789/6129
_version_ 1645151814818988032
score 13.222552

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