Natural Insoluble Materials as Potential Support for Enzyme Immobilization
Enzyme is the heart of biotechnological processes as it permits 'green' synthesis at mild working conditions and consumes less energy. However, enzyme activities are limited by deleterious effects and extreme conditions. The need to produce or design enzyme with high activities and stab...
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| Format: | Conference Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://ddms.usim.edu.my/handle/123456789/6129 |
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| Summary: | Enzyme is the heart of biotechnological processes as it permits 'green' synthesis at mild
working conditions and consumes less energy. However, enzyme activities are limited by
deleterious effects and extreme conditions. The need to produce or design enzyme with
high activities and stabilities for the green synthesis is therefore, a new challenge for
scientists. In this study, the effects of natural insoluble materials, mainly plagioclase
feldspar, mica and fibres of kenalf, were extensively explored for their use as support for
enzyme immobilization. The use of these materials is advantageous as they can be found
in abundant, possess good physico-chemical properties and are least expensive.
Following a simple and straightforward, physical adsorption technique of immobilization,
the natural materials exhibited high enzyme loading of 60-90% and enhanced enzyme
activity by 4-8 folds, when tested in the syntheses of fine chemicals. Enzyme activities
and stabilities were retained even after long exposure (10 days) to extremely polar organic
solvents (log P = -0.3), extreme storage conditions and high temperature (70 °C). The
immobilized enzymes also showed promising recyclability and kinetic profile as compared
to native enzyme. The enzyme preparations produced has displayed promising action as
biocatalysts for industrial applications. |
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