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Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts

Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic...

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Main Authors: Yang, Shun Kai, Leo, Teik Kee, Mai, Chun Wai, Chin, Swee Yee, Lamasudin, Dhilia Udie, Lim, Erin Swee Hua, Tan, Ngai Paing, Lai, Kok Song
Format: Article
Language:English
Published: Wolters Kluwer 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87346/1/Protein%20expression%20patterns%20in%20HEK-.pdf
http://psasir.upm.edu.my/id/eprint/87346/
https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=70;spage=431;epage=434;aulast=Yang;type=0
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spelling my.upm.eprints.873462022-11-07T09:09:04Z http://psasir.upm.edu.my/id/eprint/87346/ Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts Yang, Shun Kai Leo, Teik Kee Mai, Chun Wai Chin, Swee Yee Lamasudin, Dhilia Udie Lim, Erin Swee Hua Tan, Ngai Paing Lai, Kok Song Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. Wolters Kluwer 2020-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87346/1/Protein%20expression%20patterns%20in%20HEK-.pdf Yang, Shun Kai and Leo, Teik Kee and Mai, Chun Wai and Chin, Swee Yee and Lamasudin, Dhilia Udie and Lim, Erin Swee Hua and Tan, Ngai Paing and Lai, Kok Song (2020) Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts. Pharmacognosy Magazine, 70 (16). 431 - 434. ISSN 0973-1296; ESSN: 0976-4062 https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=70;spage=431;epage=434;aulast=Yang;type=0 10.4103/pm.pm_281_19
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process.
format Article
author Yang, Shun Kai
Leo, Teik Kee
Mai, Chun Wai
Chin, Swee Yee
Lamasudin, Dhilia Udie
Lim, Erin Swee Hua
Tan, Ngai Paing
Lai, Kok Song
spellingShingle Yang, Shun Kai
Leo, Teik Kee
Mai, Chun Wai
Chin, Swee Yee
Lamasudin, Dhilia Udie
Lim, Erin Swee Hua
Tan, Ngai Paing
Lai, Kok Song
Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
author_facet Yang, Shun Kai
Leo, Teik Kee
Mai, Chun Wai
Chin, Swee Yee
Lamasudin, Dhilia Udie
Lim, Erin Swee Hua
Tan, Ngai Paing
Lai, Kok Song
author_sort Yang, Shun Kai
title Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
title_short Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
title_full Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
title_fullStr Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
title_full_unstemmed Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
title_sort protein expression patterns in hek-blue-cells treated with clinacanthus nutans extracts
publisher Wolters Kluwer
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87346/1/Protein%20expression%20patterns%20in%20HEK-.pdf
http://psasir.upm.edu.my/id/eprint/87346/
https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=70;spage=431;epage=434;aulast=Yang;type=0
_version_ 1751538211677536256
score 13.211869

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