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Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia

Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2...

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Main Author: Hussein, Nasreldin Elhadi
Format: Thesis
Language:English
English
Published: 1999
Online Access:http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf
http://psasir.upm.edu.my/id/eprint/8411/
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spelling my.upm.eprints.84112012-07-03T04:42:58Z http://psasir.upm.edu.my/id/eprint/8411/ Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia Hussein, Nasreldin Elhadi Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2% of the isolates) and 2 (27.8%). Twenty one strains of biotypes 1 and 2 harboured plasmid DNA ranging in size from 1.4 to 9.7 megaDalton. No particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers demonstrated polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotypes 1 and 2 of the V vulnificus strains investigated. RAPD identity across biotypes was also observed among the V vulnificus strains. 35 Vibrio parahaemolyticus Kanagawa-negative strains were isolated.· Twenty six strains of V parahaemolyticus were carried small plasmid(s) of 1.3 to 9.7 MegaDalton that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The RAPD fingerprinting using three primers demonstrated polymorphisms in all thirty-five strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the V. parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. 1999 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf Hussein, Nasreldin Elhadi (1999) Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2% of the isolates) and 2 (27.8%). Twenty one strains of biotypes 1 and 2 harboured plasmid DNA ranging in size from 1.4 to 9.7 megaDalton. No particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers demonstrated polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotypes 1 and 2 of the V vulnificus strains investigated. RAPD identity across biotypes was also observed among the V vulnificus strains. 35 Vibrio parahaemolyticus Kanagawa-negative strains were isolated.· Twenty six strains of V parahaemolyticus were carried small plasmid(s) of 1.3 to 9.7 MegaDalton that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The RAPD fingerprinting using three primers demonstrated polymorphisms in all thirty-five strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the V. parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus.
format Thesis
author Hussein, Nasreldin Elhadi
spellingShingle Hussein, Nasreldin Elhadi
Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
author_facet Hussein, Nasreldin Elhadi
author_sort Hussein, Nasreldin Elhadi
title Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_short Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_full Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_fullStr Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_full_unstemmed Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_sort isolation and molecular characterisation of vibrio vulnificus and vibrio parahaemolyticus from cockles (anadara granosa) in malaysia
publishDate 1999
url http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf
http://psasir.upm.edu.my/id/eprint/8411/
_version_ 1643824009110880256
score 13.211869

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