Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf http://psasir.upm.edu.my/id/eprint/8411/ |
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| Summary: | Antibiotic susceptibility, plasmid profiles and random amplification of
polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio
parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus
isolates were examined. The prevalent biotype was biotype 1 (72.2% of the isolates) and 2
(27.8%). Twenty one strains of biotypes 1 and 2 harboured plasmid DNA ranging in size
from 1.4 to 9.7 megaDalton. No particular plasmid profile was predictive of a particular
pattern of antibiotic susceptibility. Two primers demonstrated polymorphisms in all
strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability
among both biotypes 1 and 2 of the V vulnificus strains investigated. RAPD identity
across biotypes was also observed among the V vulnificus strains. 35 Vibrio
parahaemolyticus Kanagawa-negative strains were isolated.· Twenty six strains of V
parahaemolyticus were carried small plasmid(s) of 1.3 to 9.7 MegaDalton that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The RAPD
fingerprinting using three primers demonstrated polymorphisms in all thirty-five strains
of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD
profiles revealed a high level of DNA sequence diversity within the V. parahaemolyticus
strains tested, and that cockles in the study area are populated by genetically polymorphic
strains of V. parahaemolyticus. |
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