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L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase

Enzymatic resolution of DL-amino acids using aminoacylase immobilised on DEAE-sephadex has been employed in the industry for the production of the optically active, L-amino acid There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisatio...

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Main Author: Siaw, Yew Seong
Format: Thesis
Language:English
English
Published: 1993
Online Access:http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf
http://psasir.upm.edu.my/id/eprint/8358/
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spelling my.upm.eprints.83582012-11-02T02:12:32Z http://psasir.upm.edu.my/id/eprint/8358/ L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase Siaw, Yew Seong Enzymatic resolution of DL-amino acids using aminoacylase immobilised on DEAE-sephadex has been employed in the industry for the production of the optically active, L-amino acid There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisation method for aminoacylase and to report the preparation, properties, and applicability of the resulting immobilised aminoacylase. Aminoacylase was immobilised in calcium alginate beads(ACAG). The ACAG was further stabilised by coating with polyethyleneimine (PEI), a mixture of polyethyleneimine and glutaraldehyde(PEI-GLU), poly-L-lysine (PLL), or by cross - linking with carbodiimide. Each type of the immobilised aminoacylase was then used to perform the optical resolution of N-acetyl-DL-phenylalanine. 1993 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf Siaw, Yew Seong (1993) L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase. Masters thesis, Universiti Pertanian Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Enzymatic resolution of DL-amino acids using aminoacylase immobilised on DEAE-sephadex has been employed in the industry for the production of the optically active, L-amino acid There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisation method for aminoacylase and to report the preparation, properties, and applicability of the resulting immobilised aminoacylase. Aminoacylase was immobilised in calcium alginate beads(ACAG). The ACAG was further stabilised by coating with polyethyleneimine (PEI), a mixture of polyethyleneimine and glutaraldehyde(PEI-GLU), poly-L-lysine (PLL), or by cross - linking with carbodiimide. Each type of the immobilised aminoacylase was then used to perform the optical resolution of N-acetyl-DL-phenylalanine.
format Thesis
author Siaw, Yew Seong
spellingShingle Siaw, Yew Seong
L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
author_facet Siaw, Yew Seong
author_sort Siaw, Yew Seong
title L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
title_short L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
title_full L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
title_fullStr L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
title_full_unstemmed L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
title_sort l-phenylalanine production by stabilised calcium alginate immobilised aminoacylase
publishDate 1993
url http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf
http://psasir.upm.edu.my/id/eprint/8358/
_version_ 1643823993211322368
score 13.211869

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