L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
Enzymatic resolution of DL-amino acids using aminoacylase immobilised on DEAE-sephadex has been employed in the industry for the production of the optically active, L-amino acid There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisatio...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf http://psasir.upm.edu.my/id/eprint/8358/ |
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| Summary: | Enzymatic resolution of DL-amino acids using aminoacylase
immobilised on DEAE-sephadex has been employed in the industry
for the production of the optically active, L-amino acid
There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisation method for aminoacylase and to report the
preparation, properties, and applicability of the resulting
immobilised aminoacylase. Aminoacylase was immobilised in
calcium alginate beads(ACAG). The ACAG was further stabilised
by coating with polyethyleneimine (PEI), a mixture of
polyethyleneimine and glutaraldehyde(PEI-GLU), poly-L-lysine
(PLL), or by cross - linking with carbodiimide. Each type of the
immobilised aminoacylase was then used to perform the optical
resolution of N-acetyl-DL-phenylalanine. |
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