Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology

Seeds of red pitaya fruit (Hylocereus polyrhizus) are commonly left underutilized in the food industry. Thus, the objective of this present study was to optimize the extraction condition for the maximum DPPH radical scavenging activity of red pitaya fruit seed extract using response surface meth...

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Main Authors: Zulkifli, Siti Atikah, Abd Gani, Siti Salwa, Zaidan, Uswatun Hasanah, Halmi, Mohd Izuan Effendi
Format: Article
Language:English
Published: Blue Eyes Intelligence Engineering & Sciences Publication 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81561/1/Optimization%20of%20antioxidant%20extraction%20in%20hylocereus%20polyrhizus%20seed%20using%20response%20surface%20methodology.pdf
http://psasir.upm.edu.my/id/eprint/81561/
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spelling my.upm.eprints.815612020-10-29T19:04:56Z http://psasir.upm.edu.my/id/eprint/81561/ Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology Zulkifli, Siti Atikah Abd Gani, Siti Salwa Zaidan, Uswatun Hasanah Halmi, Mohd Izuan Effendi Seeds of red pitaya fruit (Hylocereus polyrhizus) are commonly left underutilized in the food industry. Thus, the objective of this present study was to optimize the extraction condition for the maximum DPPH radical scavenging activity of red pitaya fruit seed extract using response surface methodology (RSM). A Central Composite design was employed to determine the effects of the selected variables, comprising extraction time (30-90 min), extraction temperature (40-80 ℃) and ethanol concentration (60-80 %) on the DPPH scavenging activities. Data were analyzed by using Design Expert (version 10.0.1, Stat-Ease, Inc., Minneapolis, MN, USA) statistical analysis software. The optimal extraction conditions for the highest antioxidant capacity were derived at 45 mins of extraction time, 70℃ extraction temperature and 80 % ethanol concentration that resulted in 92.89 % of scavenging activity. The optimized model developed was verified by comparing the predicted and experimental value of response. The result of measured response agreed well with the predicted values, demonstrating that the model can be used for optimizing the conditions of RSP extract that ensure high recovery of DPPH radical scavenging activity. Blue Eyes Intelligence Engineering & Sciences Publication 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81561/1/Optimization%20of%20antioxidant%20extraction%20in%20hylocereus%20polyrhizus%20seed%20using%20response%20surface%20methodology.pdf Zulkifli, Siti Atikah and Abd Gani, Siti Salwa and Zaidan, Uswatun Hasanah and Halmi, Mohd Izuan Effendi (2019) Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology. International Journal of Engineering and Advanced Technology (IJEAT), 8 (6S3). pp. 43-47. ISSN 2249-8958 10.35940/ijeat.F1007.0986S319
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Seeds of red pitaya fruit (Hylocereus polyrhizus) are commonly left underutilized in the food industry. Thus, the objective of this present study was to optimize the extraction condition for the maximum DPPH radical scavenging activity of red pitaya fruit seed extract using response surface methodology (RSM). A Central Composite design was employed to determine the effects of the selected variables, comprising extraction time (30-90 min), extraction temperature (40-80 ℃) and ethanol concentration (60-80 %) on the DPPH scavenging activities. Data were analyzed by using Design Expert (version 10.0.1, Stat-Ease, Inc., Minneapolis, MN, USA) statistical analysis software. The optimal extraction conditions for the highest antioxidant capacity were derived at 45 mins of extraction time, 70℃ extraction temperature and 80 % ethanol concentration that resulted in 92.89 % of scavenging activity. The optimized model developed was verified by comparing the predicted and experimental value of response. The result of measured response agreed well with the predicted values, demonstrating that the model can be used for optimizing the conditions of RSP extract that ensure high recovery of DPPH radical scavenging activity.
format Article
author Zulkifli, Siti Atikah
Abd Gani, Siti Salwa
Zaidan, Uswatun Hasanah
Halmi, Mohd Izuan Effendi
spellingShingle Zulkifli, Siti Atikah
Abd Gani, Siti Salwa
Zaidan, Uswatun Hasanah
Halmi, Mohd Izuan Effendi
Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
author_facet Zulkifli, Siti Atikah
Abd Gani, Siti Salwa
Zaidan, Uswatun Hasanah
Halmi, Mohd Izuan Effendi
author_sort Zulkifli, Siti Atikah
title Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
title_short Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
title_full Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
title_fullStr Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
title_full_unstemmed Optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
title_sort optimization of antioxidant extraction in hylocereus polyrhizus seed using response surface methodology
publisher Blue Eyes Intelligence Engineering & Sciences Publication
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81561/1/Optimization%20of%20antioxidant%20extraction%20in%20hylocereus%20polyrhizus%20seed%20using%20response%20surface%20methodology.pdf
http://psasir.upm.edu.my/id/eprint/81561/
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score 13.211869