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Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate

The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed by Raw Starch Degrading Enzyme (RSDE). In order to enhance its hydrolysability, the starch was treated with acid and heated below its gelatinization temperature, thus increasing the accessibility of t...

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Main Authors: Marlida, Yetti, Saari, Nazamid, Karim, Roselina, Mohammed, Abdulkarim Sabo
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2007
Online Access:http://psasir.upm.edu.my/id/eprint/789/1/83-90.pdf
http://psasir.upm.edu.my/id/eprint/789/
http://www.ifrj.upm.edu.my/afjv14%282%292007/83-90.pdf
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spelling my.upm.eprints.7892016-11-15T07:07:52Z http://psasir.upm.edu.my/id/eprint/789/ Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate Marlida, Yetti Saari, Nazamid Karim, Roselina Mohammed, Abdulkarim Sabo The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed by Raw Starch Degrading Enzyme (RSDE). In order to enhance its hydrolysability, the starch was treated with acid and heated below its gelatinization temperature, thus increasing the accessibility of the sago starch granule to enzymatic attack. Results showed that treatment of sago starch with acid at pH 2.0 and temperature 65oC for 2 hours greatly enhanced its conversion rate to glucose from 53.3% to 71.9%. It is clearly shown that high yield of glucose is produced during hydrolysis of acid-treated sago starch using the Raw Starch Degrading Enzyme from Acremonium sp. The difference between the acid-treated and untreated sago starch in this study could be due to the differences on the surface of the sago starch granule which may influence the accessibility and diffusion of enzyme into the starch during hydrolysis. Universiti Putra Malaysia Press 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/789/1/83-90.pdf Marlida, Yetti and Saari, Nazamid and Karim, Roselina and Mohammed, Abdulkarim Sabo (2007) Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate. ASEAN Food Journal, 14 (2). pp. 83-90. ISSN 0127-7324; ESSN: 1505-5337 http://www.ifrj.upm.edu.my/afjv14%282%292007/83-90.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed by Raw Starch Degrading Enzyme (RSDE). In order to enhance its hydrolysability, the starch was treated with acid and heated below its gelatinization temperature, thus increasing the accessibility of the sago starch granule to enzymatic attack. Results showed that treatment of sago starch with acid at pH 2.0 and temperature 65oC for 2 hours greatly enhanced its conversion rate to glucose from 53.3% to 71.9%. It is clearly shown that high yield of glucose is produced during hydrolysis of acid-treated sago starch using the Raw Starch Degrading Enzyme from Acremonium sp. The difference between the acid-treated and untreated sago starch in this study could be due to the differences on the surface of the sago starch granule which may influence the accessibility and diffusion of enzyme into the starch during hydrolysis.
format Article
author Marlida, Yetti
Saari, Nazamid
Karim, Roselina
Mohammed, Abdulkarim Sabo
spellingShingle Marlida, Yetti
Saari, Nazamid
Karim, Roselina
Mohammed, Abdulkarim Sabo
Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
author_facet Marlida, Yetti
Saari, Nazamid
Karim, Roselina
Mohammed, Abdulkarim Sabo
author_sort Marlida, Yetti
title Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
title_short Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
title_full Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
title_fullStr Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
title_full_unstemmed Improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
title_sort improvement of glucose production by raw starch degrading enzyme utilizing acid-treated sago starch as substrate
publisher Universiti Putra Malaysia Press
publishDate 2007
url http://psasir.upm.edu.my/id/eprint/789/1/83-90.pdf
http://psasir.upm.edu.my/id/eprint/789/
http://www.ifrj.upm.edu.my/afjv14%282%292007/83-90.pdf
_version_ 1643821908897038336
score 13.211869

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