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Between horned and polled, is there a karyotypic difference in sheep?

A karyotypic analysis was carried out on two breeds of sheep,Merino (horned) and Dorper (polled) to compare their chromosome features. Lymphocytes were extracted from blood samples and cultured in RPMI 1640 as a culture medium, supplemented with foetal bovine serum and penicillin-streptomycin....

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Jamaluddin, Nurul Liyana
التنسيق: Project Paper Report
اللغة:English
منشور في: 2017
الوصول للمادة أونلاين:http://psasir.upm.edu.my/id/eprint/78305/1/FPV%202017%2049%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/78305/
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id my.upm.eprints.78305
record_format eprints
spelling my.upm.eprints.783052024-10-10T01:25:23Z http://psasir.upm.edu.my/id/eprint/78305/ Between horned and polled, is there a karyotypic difference in sheep? Jamaluddin, Nurul Liyana A karyotypic analysis was carried out on two breeds of sheep,Merino (horned) and Dorper (polled) to compare their chromosome features. Lymphocytes were extracted from blood samples and cultured in RPMI 1640 as a culture medium, supplemented with foetal bovine serum and penicillin-streptomycin. The cultures were made in duplicates, using phytohemagglutinin (PHA) and pokeweed (PWM) mitogens to stimulate lymphocytes growth. Cultures with PWM produce better quality and quantity of metaphase spreads compared with PHA. Karyotypes were constructed in accordance with the standard procedure of International Cytogenetic Nomenclature of Domestic Animal (ICNDA). The domestic sheep has a diploid number (2n)of 54. The chromosomes morphology of Merino and Dorper are indistinguishable based on their conventional karyotypes established. Both males and females have karyotypes of three pairs of submetacentric and 23 pairs of acrocentric autosomes. The X chromosome is identified as the largest acrocentric while the Y chromosome is identified as the smallest chromosome. The fundamental number (NF) for the male sheep is 60 and the female is also 60. 2017-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/78305/1/FPV%202017%2049%20-%20IR.pdf Jamaluddin, Nurul Liyana (2017) Between horned and polled, is there a karyotypic difference in sheep? [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A karyotypic analysis was carried out on two breeds of sheep,Merino (horned) and Dorper (polled) to compare their chromosome features. Lymphocytes were extracted from blood samples and cultured in RPMI 1640 as a culture medium, supplemented with foetal bovine serum and penicillin-streptomycin. The cultures were made in duplicates, using phytohemagglutinin (PHA) and pokeweed (PWM) mitogens to stimulate lymphocytes growth. Cultures with PWM produce better quality and quantity of metaphase spreads compared with PHA. Karyotypes were constructed in accordance with the standard procedure of International Cytogenetic Nomenclature of Domestic Animal (ICNDA). The domestic sheep has a diploid number (2n)of 54. The chromosomes morphology of Merino and Dorper are indistinguishable based on their conventional karyotypes established. Both males and females have karyotypes of three pairs of submetacentric and 23 pairs of acrocentric autosomes. The X chromosome is identified as the largest acrocentric while the Y chromosome is identified as the smallest chromosome. The fundamental number (NF) for the male sheep is 60 and the female is also 60.
format Project Paper Report
author Jamaluddin, Nurul Liyana
spellingShingle Jamaluddin, Nurul Liyana
Between horned and polled, is there a karyotypic difference in sheep?
author_facet Jamaluddin, Nurul Liyana
author_sort Jamaluddin, Nurul Liyana
title Between horned and polled, is there a karyotypic difference in sheep?
title_short Between horned and polled, is there a karyotypic difference in sheep?
title_full Between horned and polled, is there a karyotypic difference in sheep?
title_fullStr Between horned and polled, is there a karyotypic difference in sheep?
title_full_unstemmed Between horned and polled, is there a karyotypic difference in sheep?
title_sort between horned and polled, is there a karyotypic difference in sheep?
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/78305/1/FPV%202017%2049%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/78305/
_version_ 1814054588785885184
score 13.251813

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