Between horned and polled, is there a karyotypic difference in sheep?
A karyotypic analysis was carried out on two breeds of sheep,Merino (horned) and Dorper (polled) to compare their chromosome features. Lymphocytes were extracted from blood samples and cultured in RPMI 1640 as a culture medium, supplemented with foetal bovine serum and penicillin-streptomycin....
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78305/1/FPV%202017%2049%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/78305/ |
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| Summary: | A karyotypic analysis was carried out on two breeds of sheep,Merino
(horned) and Dorper (polled) to compare their chromosome features. Lymphocytes
were extracted from blood samples and cultured in RPMI 1640 as a culture medium,
supplemented with foetal bovine serum and penicillin-streptomycin. The cultures
were made in duplicates, using phytohemagglutinin (PHA) and pokeweed (PWM)
mitogens to stimulate lymphocytes growth. Cultures with PWM produce better
quality and quantity of metaphase spreads compared with PHA. Karyotypes were
constructed in accordance with the standard procedure of International Cytogenetic
Nomenclature of Domestic Animal (ICNDA). The domestic sheep has a diploid
number (2n)of 54. The chromosomes morphology of Merino and Dorper are indistinguishable based on their conventional karyotypes established. Both males
and females have karyotypes of three pairs of submetacentric and 23 pairs of
acrocentric autosomes. The X chromosome is identified as the largest acrocentric
while the Y chromosome is identified as the smallest chromosome. The fundamental
number (NF) for the male sheep is 60 and the female is also 60. |
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