Cover Image

Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules

Background: The pharmaceutical industry has boosted gelatin consumption worldwide. This is supported by the availability of cost-effective gelatin production from porcine by-products. However, cross-contamination of gelatin materials, where porcine gelatin was unintentionally included in the other a...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohamad, N. A., Mustafa, S., Khairil Mokhtar, N. F., El Sheikha, A. F.
Format: Article
Language:English
Published: Wiley 2018
Online Access:http://psasir.upm.edu.my/id/eprint/75014/1/Molecular%20beacon-based%20real-time%20PCR%20method%20for%20detection%20of%20porcine%20DNA%20in%20gelatin%20and%20gelatin%20capsules.pdf
http://psasir.upm.edu.my/id/eprint/75014/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.75014
record_format eprints
spelling my.upm.eprints.750142020-04-20T13:57:30Z http://psasir.upm.edu.my/id/eprint/75014/ Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules Mohamad, N. A. Mustafa, S. Khairil Mokhtar, N. F. El Sheikha, A. F. Background: The pharmaceutical industry has boosted gelatin consumption worldwide. This is supported by the availability of cost-effective gelatin production from porcine by-products. However, cross-contamination of gelatin materials, where porcine gelatin was unintentionally included in the other animal sources of gelatin, has caused significant concerns about halal authenticity. The real-time polymerase chain reaction (PCR) has enabled a highly specific and sensitive animal species detection method in various food products. Hence, such a technique was employed in the present study to detect and quantify porcine DNA in gelatin using a molecular beacon probe, with differences in performance between mitochondrial (cytochrome b gene) and chromosomal DNA-(MPRE42 repetitive element) based porcine-specific PCR assays being compared. Results: A higher sensitivity was observed in chromosomal DNA (MPRE-PCR assay), where this assay allows the detection of gelatin DNA at amounts as as low as 1 pg, whereas mitochondrial DNA (CBH-PCR assay) can only detect at levels down to 10 pg of gelatin DNA. When an analysis with commercial gelatin and gelatin capsule samples was conducted, the same result was observed, with a significantly more sensitive detection being provided by the repetitive element of chromosomal DNA. Conclusion: The present study has established highly sensitive DNA-based porcine detection systems derived from chromosomal DNA that are feasible for highly processed products such as gelatin and gelatin capsules containing a minute amount of DNA. This sensitive detection method can also be implemented to assist the halal authentication process of various food products available on the market. Wiley 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75014/1/Molecular%20beacon-based%20real-time%20PCR%20method%20for%20detection%20of%20porcine%20DNA%20in%20gelatin%20and%20gelatin%20capsules.pdf Mohamad, N. A. and Mustafa, S. and Khairil Mokhtar, N. F. and El Sheikha, A. F. (2018) Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules. Journal of the Science of Food and Agriculture, 98 (12). 4570 - 4577. ISSN 0022-5142; ESSN: 1097-0010 10.1002/jsfa.8985.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: The pharmaceutical industry has boosted gelatin consumption worldwide. This is supported by the availability of cost-effective gelatin production from porcine by-products. However, cross-contamination of gelatin materials, where porcine gelatin was unintentionally included in the other animal sources of gelatin, has caused significant concerns about halal authenticity. The real-time polymerase chain reaction (PCR) has enabled a highly specific and sensitive animal species detection method in various food products. Hence, such a technique was employed in the present study to detect and quantify porcine DNA in gelatin using a molecular beacon probe, with differences in performance between mitochondrial (cytochrome b gene) and chromosomal DNA-(MPRE42 repetitive element) based porcine-specific PCR assays being compared. Results: A higher sensitivity was observed in chromosomal DNA (MPRE-PCR assay), where this assay allows the detection of gelatin DNA at amounts as as low as 1 pg, whereas mitochondrial DNA (CBH-PCR assay) can only detect at levels down to 10 pg of gelatin DNA. When an analysis with commercial gelatin and gelatin capsule samples was conducted, the same result was observed, with a significantly more sensitive detection being provided by the repetitive element of chromosomal DNA. Conclusion: The present study has established highly sensitive DNA-based porcine detection systems derived from chromosomal DNA that are feasible for highly processed products such as gelatin and gelatin capsules containing a minute amount of DNA. This sensitive detection method can also be implemented to assist the halal authentication process of various food products available on the market.
format Article
author Mohamad, N. A.
Mustafa, S.
Khairil Mokhtar, N. F.
El Sheikha, A. F.
spellingShingle Mohamad, N. A.
Mustafa, S.
Khairil Mokhtar, N. F.
El Sheikha, A. F.
Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
author_facet Mohamad, N. A.
Mustafa, S.
Khairil Mokhtar, N. F.
El Sheikha, A. F.
author_sort Mohamad, N. A.
title Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
title_short Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
title_full Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
title_fullStr Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
title_full_unstemmed Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
title_sort molecular beacon-based real-time pcr method for detection of porcine dna in gelatin and gelatin capsules
publisher Wiley
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/75014/1/Molecular%20beacon-based%20real-time%20PCR%20method%20for%20detection%20of%20porcine%20DNA%20in%20gelatin%20and%20gelatin%20capsules.pdf
http://psasir.upm.edu.my/id/eprint/75014/
_version_ 1665896022917775360
score 13.211869

Similar Items