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Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics

Antibiotic resistance in campylobacter is an emerging global public health problem after MRSA and VRE. Fluoroquinolone and macrolide resistance have been found to be more common in this world leading foodborne pathogen. A total of fifty-six isolates of Campylobacter jejuni obtained from raw vegetabl...

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Main Authors: Chai, Lay Ching, Abu Bakar, Fatimah, Mohamad Ghazali, Farinazleen, Lee, Hai Yen, Robin, Tunung, Abdul Talib, Shamsinar, Ahmad Suhaimi, Laila Rabaah, Zakaria, Thahirahtul Asma, Malakar, Pradeep Kumar, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Radu, Son
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2008
Online Access:http://psasir.upm.edu.my/id/eprint/748/1/125-134.pdf
http://psasir.upm.edu.my/id/eprint/748/
http://www.ifrj.upm.edu.my/15%20%282%29%202008/125-134.pdf
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spelling my.upm.eprints.7482015-09-23T04:13:23Z http://psasir.upm.edu.my/id/eprint/748/ Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics Chai, Lay Ching Abu Bakar, Fatimah Mohamad Ghazali, Farinazleen Lee, Hai Yen Robin, Tunung Abdul Talib, Shamsinar Ahmad Suhaimi, Laila Rabaah Zakaria, Thahirahtul Asma Malakar, Pradeep Kumar Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Antibiotic resistance in campylobacter is an emerging global public health problem after MRSA and VRE. Fluoroquinolone and macrolide resistance have been found to be more common in this world leading foodborne pathogen. A total of fifty-six isolates of Campylobacter jejuni obtained from raw vegetables which are consumed as ulam (salad) in Malaysia, were tested with 12 antibiotics used clinically and agriculturally. The resistance was determined using the disk diffusion method. Results were determined by hierarchic numerical methods to cluster strains and antibiotics according to similarity profiles. Fifty five C. jejuni isolates from different isolation sites were all clustered together into ten groups. This indicates that the commodities (raw salad vegetables/ulam) where the isolates originated might share a similar source of cross-contamination along the production route. All antibiotics tested correlated and there were four groupings reflecting their mode of actions. Generally, C. jejuni isolates were found to be highly resistant to erythromycin (91.1%) and tetracycline (85.7%). Both agents are popular antibiotics used clinically to treat bacterial infections. On the other hand, the C. jejuni isolates showed high percentage (80.4%) of resistance towards enrofloxacin, an extensively used antimicrobial agent in agriculture practices. This study showed that C. jejuni isolates were highly multi-resistance to as many as 10 antibiotics. Therefore, in terms of biosafety, the presence of antibiotic resistance strains in the food chain has raised concerns that the treatment of human infections will be compromised. Faculty of Food Science and Technology, Universiti Putra Malaysia 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/748/1/125-134.pdf Chai, Lay Ching and Abu Bakar, Fatimah and Mohamad Ghazali, Farinazleen and Lee, Hai Yen and Robin, Tunung and Abdul Talib, Shamsinar and Ahmad Suhaimi, Laila Rabaah and Zakaria, Thahirahtul Asma and Malakar, Pradeep Kumar and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2008) Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics. International Food Research Journal, 15 (2). pp. 125-134. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/15%20%282%29%202008/125-134.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Antibiotic resistance in campylobacter is an emerging global public health problem after MRSA and VRE. Fluoroquinolone and macrolide resistance have been found to be more common in this world leading foodborne pathogen. A total of fifty-six isolates of Campylobacter jejuni obtained from raw vegetables which are consumed as ulam (salad) in Malaysia, were tested with 12 antibiotics used clinically and agriculturally. The resistance was determined using the disk diffusion method. Results were determined by hierarchic numerical methods to cluster strains and antibiotics according to similarity profiles. Fifty five C. jejuni isolates from different isolation sites were all clustered together into ten groups. This indicates that the commodities (raw salad vegetables/ulam) where the isolates originated might share a similar source of cross-contamination along the production route. All antibiotics tested correlated and there were four groupings reflecting their mode of actions. Generally, C. jejuni isolates were found to be highly resistant to erythromycin (91.1%) and tetracycline (85.7%). Both agents are popular antibiotics used clinically to treat bacterial infections. On the other hand, the C. jejuni isolates showed high percentage (80.4%) of resistance towards enrofloxacin, an extensively used antimicrobial agent in agriculture practices. This study showed that C. jejuni isolates were highly multi-resistance to as many as 10 antibiotics. Therefore, in terms of biosafety, the presence of antibiotic resistance strains in the food chain has raised concerns that the treatment of human infections will be compromised.
format Article
author Chai, Lay Ching
Abu Bakar, Fatimah
Mohamad Ghazali, Farinazleen
Lee, Hai Yen
Robin, Tunung
Abdul Talib, Shamsinar
Ahmad Suhaimi, Laila Rabaah
Zakaria, Thahirahtul Asma
Malakar, Pradeep Kumar
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
spellingShingle Chai, Lay Ching
Abu Bakar, Fatimah
Mohamad Ghazali, Farinazleen
Lee, Hai Yen
Robin, Tunung
Abdul Talib, Shamsinar
Ahmad Suhaimi, Laila Rabaah
Zakaria, Thahirahtul Asma
Malakar, Pradeep Kumar
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
author_facet Chai, Lay Ching
Abu Bakar, Fatimah
Mohamad Ghazali, Farinazleen
Lee, Hai Yen
Robin, Tunung
Abdul Talib, Shamsinar
Ahmad Suhaimi, Laila Rabaah
Zakaria, Thahirahtul Asma
Malakar, Pradeep Kumar
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
author_sort Chai, Lay Ching
title Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
title_short Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
title_full Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
title_fullStr Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
title_full_unstemmed Biosafety of Campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
title_sort biosafety of campylobacter jejuni from raw vegetables consumed as ulam with reference to their resistance to antibiotics
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/748/1/125-134.pdf
http://psasir.upm.edu.my/id/eprint/748/
http://www.ifrj.upm.edu.my/15%20%282%29%202008/125-134.pdf
_version_ 1643821903351119872
score 13.211869

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