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Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia

Peanuts are widely consumed as the main ingredient in many local dishes in Malaysia. However, the tropical climate in Malaysia (high temperature and humidity) favours the growth of fungi from Aspergillus section Flavi, especially during storage. Most of the species from this section, such as A. flav...

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Main Authors: Mahror, Norlia, Selamat, Jinap, Mahmud @ Ab Rashid, Nor Khaizura, Radu, Son, Chin, Cheow Keat, Sardjono
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73549/1/Polyphasic%20approach%20to%20the%20identification%20and%20characterization%20of%20aflatoxigenic%20strains%20of%20Aspergillus%20section%20Flavi%20isolated%20from%20peanuts%20and%20peanut-based%20products%20marketed%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/73549/
https://www.sciencedirect.com/science/article/pii/S0168160518302952
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spelling my.upm.eprints.735492020-07-06T01:14:17Z http://psasir.upm.edu.my/id/eprint/73549/ Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia Mahror, Norlia Selamat, Jinap Mahmud @ Ab Rashid, Nor Khaizura Radu, Son Chin, Cheow Keat Sardjono Peanuts are widely consumed as the main ingredient in many local dishes in Malaysia. However, the tropical climate in Malaysia (high temperature and humidity) favours the growth of fungi from Aspergillus section Flavi, especially during storage. Most of the species from this section, such as A. flavus, A. parasiticus and A. nomius, are natural producers of aflatoxins. Precise identification of local isolates and information regarding their ability to produce aflatoxins are very important to evaluate the safety of food marketed in Malaysia. Therefore, this study aimed to identify and characterize the aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi in peanuts and peanut-based products. A polyphasic approach, consisting of morphological and chemical characterizations was applied to 128 isolates originating from raw peanuts and peanut-based products. On the basis of morphological characters, 127 positively identified as Aspergillus flavus, and the other as A. nomius. Chemical characterization revealed six chemotype profiles which indicates diversity of toxigenic potential. About 58.6%, 68.5%, and 100% of the isolates are positive for aflatoxins, cyclopiazonic acid and aspergillic acid productions respectively. The majority of the isolates originating from raw peanut samples (64.8%) were aflatoxigenic, while those from peanut-based products were less toxigenic (39.1%). The precise identification of these species may help in developing control strategies for aflatoxigenic fungi and aflatoxin contamination in peanuts, especially during storage. These findings also highlight the possibility of the co-occurrence of other toxins, which could increase the potential toxic effects of peanuts. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73549/1/Polyphasic%20approach%20to%20the%20identification%20and%20characterization%20of%20aflatoxigenic%20strains%20of%20Aspergillus%20section%20Flavi%20isolated%20from%20peanuts%20and%20peanut-based%20products%20marketed%20in%20Malaysia.pdf Mahror, Norlia and Selamat, Jinap and Mahmud @ Ab Rashid, Nor Khaizura and Radu, Son and Chin, Cheow Keat and Sardjono (2018) Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia. International Journal of Food Microbiology, 282. pp. 9-15. ISSN 0168-1605; ESSN: 1879-3460 https://www.sciencedirect.com/science/article/pii/S0168160518302952 10.1016/j.ijfoodmicro.2018.05.030
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Peanuts are widely consumed as the main ingredient in many local dishes in Malaysia. However, the tropical climate in Malaysia (high temperature and humidity) favours the growth of fungi from Aspergillus section Flavi, especially during storage. Most of the species from this section, such as A. flavus, A. parasiticus and A. nomius, are natural producers of aflatoxins. Precise identification of local isolates and information regarding their ability to produce aflatoxins are very important to evaluate the safety of food marketed in Malaysia. Therefore, this study aimed to identify and characterize the aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi in peanuts and peanut-based products. A polyphasic approach, consisting of morphological and chemical characterizations was applied to 128 isolates originating from raw peanuts and peanut-based products. On the basis of morphological characters, 127 positively identified as Aspergillus flavus, and the other as A. nomius. Chemical characterization revealed six chemotype profiles which indicates diversity of toxigenic potential. About 58.6%, 68.5%, and 100% of the isolates are positive for aflatoxins, cyclopiazonic acid and aspergillic acid productions respectively. The majority of the isolates originating from raw peanut samples (64.8%) were aflatoxigenic, while those from peanut-based products were less toxigenic (39.1%). The precise identification of these species may help in developing control strategies for aflatoxigenic fungi and aflatoxin contamination in peanuts, especially during storage. These findings also highlight the possibility of the co-occurrence of other toxins, which could increase the potential toxic effects of peanuts.
format Article
author Mahror, Norlia
Selamat, Jinap
Mahmud @ Ab Rashid, Nor Khaizura
Radu, Son
Chin, Cheow Keat
Sardjono
spellingShingle Mahror, Norlia
Selamat, Jinap
Mahmud @ Ab Rashid, Nor Khaizura
Radu, Son
Chin, Cheow Keat
Sardjono
Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia
author_facet Mahror, Norlia
Selamat, Jinap
Mahmud @ Ab Rashid, Nor Khaizura
Radu, Son
Chin, Cheow Keat
Sardjono
author_sort Mahror, Norlia
title Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia
title_short Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia
title_full Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia
title_fullStr Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia
title_full_unstemmed Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products marketed in Malaysia
title_sort polyphasic approach to the identification and characterization of aflatoxigenic strains of aspergillus section flavi isolated from peanuts and peanut-based products marketed in malaysia
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73549/1/Polyphasic%20approach%20to%20the%20identification%20and%20characterization%20of%20aflatoxigenic%20strains%20of%20Aspergillus%20section%20Flavi%20isolated%20from%20peanuts%20and%20peanut-based%20products%20marketed%20in%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/73549/
https://www.sciencedirect.com/science/article/pii/S0168160518302952
_version_ 1672612207215509504
score 13.211869

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