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Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration

Membrane technology has been successfully applied for the purification of bromelain, a protease enzyme from pineapple. However, the current system operates less optimally in terms of flux and separation primarily due to properties of the feed, such as viscosity. Hence, in this study, enzymatic pretr...

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Main Authors: Mohd Nor, Mohd Zuhair, Ramchandran, Lata, Duke, Mikel, Vasiljevic, Todor
Format: Article
Language:English
Published: University of Zagreb 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73469/1/Performance%20of%20a%20two-stage%20membrane%20system%20for%20bromelain%20separation%20from%20pineapple%20waste%20mixture%20as%20impacted%20by%20enzymatic%20pretreatment%20and%20diafiltration.pdf
http://psasir.upm.edu.my/id/eprint/73469/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117993/
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spelling my.upm.eprints.734692020-06-08T00:26:26Z http://psasir.upm.edu.my/id/eprint/73469/ Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration Mohd Nor, Mohd Zuhair Ramchandran, Lata Duke, Mikel Vasiljevic, Todor Membrane technology has been successfully applied for the purification of bromelain, a protease enzyme from pineapple. However, the current system operates less optimally in terms of flux and separation primarily due to properties of the feed, such as viscosity. Hence, in this study, enzymatic pretreatment and diafiltration operation were employed in a two-stage ultrafiltration (UF) system to enhance the performance of the purification and concentration process of bromelain enzyme from an extract of pineapple crude waste mixture (CWM). Pretreatment of the CWM extract using either pectinase or cellulase, or the combination of both, was applied and compared regarding the apparent viscosity reduction. Diafiltration step was introduced in UF stage 2 and observations on the flux performance, enzyme recovery and enzyme purity were made. A 12% apparent viscosity reduction was achieved when the CWM extract was pretreated with pectinase which led to 37-38% improvement in the flux performance of both UF stages, as well as higher enzyme recovery in UF stage 1. The introduction of diafiltration mode in UF stage 2 managed to sustain high flux values while yielding 4.4-fold enzyme purity (higher than a 2.5-fold purity achieved in our previous work); however, high diluent consumption was needed. The outcomes of this study showed that the flux performance and bromelain separation can be enhanced by reducing the viscosity with the employment of enzymatic pretreatment and diafiltration operation. Thus, both techniques can be potentially applied in a large-scale membrane-based process for bromelain production. University of Zagreb 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73469/1/Performance%20of%20a%20two-stage%20membrane%20system%20for%20bromelain%20separation%20from%20pineapple%20waste%20mixture%20as%20impacted%20by%20enzymatic%20pretreatment%20and%20diafiltration.pdf Mohd Nor, Mohd Zuhair and Ramchandran, Lata and Duke, Mikel and Vasiljevic, Todor (2018) Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration. Food Technology and Biotechnology, 56 (2). 218 - 227. ISSN 1330-9862 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117993/ 10.17113/ftb.56.02.18.5478
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Membrane technology has been successfully applied for the purification of bromelain, a protease enzyme from pineapple. However, the current system operates less optimally in terms of flux and separation primarily due to properties of the feed, such as viscosity. Hence, in this study, enzymatic pretreatment and diafiltration operation were employed in a two-stage ultrafiltration (UF) system to enhance the performance of the purification and concentration process of bromelain enzyme from an extract of pineapple crude waste mixture (CWM). Pretreatment of the CWM extract using either pectinase or cellulase, or the combination of both, was applied and compared regarding the apparent viscosity reduction. Diafiltration step was introduced in UF stage 2 and observations on the flux performance, enzyme recovery and enzyme purity were made. A 12% apparent viscosity reduction was achieved when the CWM extract was pretreated with pectinase which led to 37-38% improvement in the flux performance of both UF stages, as well as higher enzyme recovery in UF stage 1. The introduction of diafiltration mode in UF stage 2 managed to sustain high flux values while yielding 4.4-fold enzyme purity (higher than a 2.5-fold purity achieved in our previous work); however, high diluent consumption was needed. The outcomes of this study showed that the flux performance and bromelain separation can be enhanced by reducing the viscosity with the employment of enzymatic pretreatment and diafiltration operation. Thus, both techniques can be potentially applied in a large-scale membrane-based process for bromelain production.
format Article
author Mohd Nor, Mohd Zuhair
Ramchandran, Lata
Duke, Mikel
Vasiljevic, Todor
spellingShingle Mohd Nor, Mohd Zuhair
Ramchandran, Lata
Duke, Mikel
Vasiljevic, Todor
Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
author_facet Mohd Nor, Mohd Zuhair
Ramchandran, Lata
Duke, Mikel
Vasiljevic, Todor
author_sort Mohd Nor, Mohd Zuhair
title Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
title_short Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
title_full Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
title_fullStr Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
title_full_unstemmed Performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
title_sort performance of a two-stage membrane system for bromelain separation from pineapple waste mixture as impacted by enzymatic pretreatment and diafiltration
publisher University of Zagreb
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73469/1/Performance%20of%20a%20two-stage%20membrane%20system%20for%20bromelain%20separation%20from%20pineapple%20waste%20mixture%20as%20impacted%20by%20enzymatic%20pretreatment%20and%20diafiltration.pdf
http://psasir.upm.edu.my/id/eprint/73469/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117993/
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score 13.211869

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