Cover Image

Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)

The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)6, ds(AT)6 and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is inter...

Full description

Saved in:
Bibliographic Details
Main Authors: Seng, Hoi Ling, Ong, Alan Han Kiat, Raja Abdul Rahman, Raja Noor Zaliha, Mohd Yamin, Bohari, Tiekink, Edward R. T., Tan, Kong Wai, Maah, Mohd Jamil, Caracelli, Ignez, Ng, Chew Hee
Format: Article
Language:English
Published: Elsevier 2008
Online Access:http://psasir.upm.edu.my/id/eprint/7325/1/Factors%20affecting%20nucleolytic%20efficiency%20of%20some%20ternary%20metal%20complexes%20with%20DNA%20binding%20and%20recognition%20domains.%20Crystal%20and%20molecular%20structure%20of%20Zn%28phen%29%28edda%29.pdf
http://psasir.upm.edu.my/id/eprint/7325/
http://www.sciencedirect.com/science/article/pii/S0162013408001803
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.7325
record_format eprints
spelling my.upm.eprints.73252016-04-26T08:55:49Z http://psasir.upm.edu.my/id/eprint/7325/ Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) Seng, Hoi Ling Ong, Alan Han Kiat Raja Abdul Rahman, Raja Noor Zaliha Mohd Yamin, Bohari Tiekink, Edward R. T. Tan, Kong Wai Maah, Mohd Jamil Caracelli, Ignez Ng, Chew Hee The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)6, ds(AT)6 and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is intercalation and there is selectivity towards AT-sequence and stacking preference for A/A parallel or diagonal adjacent base steps in their intercalation. The nucleolytic properties of these complexes were investigated and the factors affecting the extent of cleavage were determined to be: concentration of complex, the nature of metal(II) ion, type of buffer, pH of buffer, incubation time, incubation temperature, and the presence of hydrogen peroxide or ascorbic acid as exogenous reagents. The fluorescence property of these complexes and its origin were also investigated. The crystal structure of the Zn(phen)(edda) complex is reported in which the zinc atom displays a distorted trans-N4O2 octahedral geometry; the crystal packing features double layers of complex molecules held together by extensive hydrogen bonding that inter-digitate with adjacent double layers via π…π interactions between 1,10-phenanthroline residues. The structure is compared with that of the recently described copper(II) analogue and, with the latter, included in molecular modeling. Elsevier 2008-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7325/1/Factors%20affecting%20nucleolytic%20efficiency%20of%20some%20ternary%20metal%20complexes%20with%20DNA%20binding%20and%20recognition%20domains.%20Crystal%20and%20molecular%20structure%20of%20Zn%28phen%29%28edda%29.pdf Seng, Hoi Ling and Ong, Alan Han Kiat and Raja Abdul Rahman, Raja Noor Zaliha and Mohd Yamin, Bohari and Tiekink, Edward R. T. and Tan, Kong Wai and Maah, Mohd Jamil and Caracelli, Ignez and Ng, Chew Hee (2008) Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda). Journal of Inorganic Biochemistry, 102 (11). pp. 1997-2011. ISSN 0162-0134; ESSN: 1873-3344 http://www.sciencedirect.com/science/article/pii/S0162013408001803 10.1016/j.jinorgbio.2008.07.015
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)6, ds(AT)6 and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is intercalation and there is selectivity towards AT-sequence and stacking preference for A/A parallel or diagonal adjacent base steps in their intercalation. The nucleolytic properties of these complexes were investigated and the factors affecting the extent of cleavage were determined to be: concentration of complex, the nature of metal(II) ion, type of buffer, pH of buffer, incubation time, incubation temperature, and the presence of hydrogen peroxide or ascorbic acid as exogenous reagents. The fluorescence property of these complexes and its origin were also investigated. The crystal structure of the Zn(phen)(edda) complex is reported in which the zinc atom displays a distorted trans-N4O2 octahedral geometry; the crystal packing features double layers of complex molecules held together by extensive hydrogen bonding that inter-digitate with adjacent double layers via π…π interactions between 1,10-phenanthroline residues. The structure is compared with that of the recently described copper(II) analogue and, with the latter, included in molecular modeling.
format Article
author Seng, Hoi Ling
Ong, Alan Han Kiat
Raja Abdul Rahman, Raja Noor Zaliha
Mohd Yamin, Bohari
Tiekink, Edward R. T.
Tan, Kong Wai
Maah, Mohd Jamil
Caracelli, Ignez
Ng, Chew Hee
spellingShingle Seng, Hoi Ling
Ong, Alan Han Kiat
Raja Abdul Rahman, Raja Noor Zaliha
Mohd Yamin, Bohari
Tiekink, Edward R. T.
Tan, Kong Wai
Maah, Mohd Jamil
Caracelli, Ignez
Ng, Chew Hee
Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
author_facet Seng, Hoi Ling
Ong, Alan Han Kiat
Raja Abdul Rahman, Raja Noor Zaliha
Mohd Yamin, Bohari
Tiekink, Edward R. T.
Tan, Kong Wai
Maah, Mohd Jamil
Caracelli, Ignez
Ng, Chew Hee
author_sort Seng, Hoi Ling
title Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
title_short Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
title_full Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
title_fullStr Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
title_full_unstemmed Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
title_sort factors affecting nucleolytic efficiency of some ternary metal complexes with dna binding and recognition domains. crystal and molecular structure of zn(phen)(edda)
publisher Elsevier
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/7325/1/Factors%20affecting%20nucleolytic%20efficiency%20of%20some%20ternary%20metal%20complexes%20with%20DNA%20binding%20and%20recognition%20domains.%20Crystal%20and%20molecular%20structure%20of%20Zn%28phen%29%28edda%29.pdf
http://psasir.upm.edu.my/id/eprint/7325/
http://www.sciencedirect.com/science/article/pii/S0162013408001803
_version_ 1643823689762865152
score 13.211869

Similar Items