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Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers

To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments...

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Main Authors: Khaledi, Kourosh Jome, Panandam, Jothi Malar, Abdul Aziz, Maheran, Siraj, Siti Shapor
Format: Article
Language:English
English
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/6568/1/Reliable%20and%20fast%20molecular%20sexing%20in%20Bos%20indicus%20cattle%20using%20amelogenin%20gene%20primers.pdf
http://psasir.upm.edu.my/id/eprint/6568/
http://www.indianjournals.com/ijor.aspx?target=ijor:jaar&volume=35&issue=1&article=017
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spelling my.upm.eprints.65682015-09-28T02:48:21Z http://psasir.upm.edu.my/id/eprint/6568/ Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers Khaledi, Kourosh Jome Panandam, Jothi Malar Abdul Aziz, Maheran Siraj, Siti Shapor To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments of 280 bp (AMELX) and 213 bp (AMELY) for the males. Thus the SE47/48 primer pair is not only reliable for sex differentiation in B. indicus but may also be used to differentiate the B. indicus and B. taurus males. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6568/1/Reliable%20and%20fast%20molecular%20sexing%20in%20Bos%20indicus%20cattle%20using%20amelogenin%20gene%20primers.pdf Khaledi, Kourosh Jome and Panandam, Jothi Malar and Abdul Aziz, Maheran and Siraj, Siti Shapor (2009) Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers. Journal of Applied Animal Research, 35 (1). pp. 83-85. ISSN 0971-2119 http://www.indianjournals.com/ijor.aspx?target=ijor:jaar&volume=35&issue=1&article=017 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments of 280 bp (AMELX) and 213 bp (AMELY) for the males. Thus the SE47/48 primer pair is not only reliable for sex differentiation in B. indicus but may also be used to differentiate the B. indicus and B. taurus males.
format Article
author Khaledi, Kourosh Jome
Panandam, Jothi Malar
Abdul Aziz, Maheran
Siraj, Siti Shapor
spellingShingle Khaledi, Kourosh Jome
Panandam, Jothi Malar
Abdul Aziz, Maheran
Siraj, Siti Shapor
Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
author_facet Khaledi, Kourosh Jome
Panandam, Jothi Malar
Abdul Aziz, Maheran
Siraj, Siti Shapor
author_sort Khaledi, Kourosh Jome
title Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
title_short Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
title_full Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
title_fullStr Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
title_full_unstemmed Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
title_sort reliable and fast molecular sexing in bos indicus cattle using amelogenin gene primers
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/6568/1/Reliable%20and%20fast%20molecular%20sexing%20in%20Bos%20indicus%20cattle%20using%20amelogenin%20gene%20primers.pdf
http://psasir.upm.edu.my/id/eprint/6568/
http://www.indianjournals.com/ijor.aspx?target=ijor:jaar&volume=35&issue=1&article=017
_version_ 1643823527821836288
score 13.211869

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