Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6568/1/Reliable%20and%20fast%20molecular%20sexing%20in%20Bos%20indicus%20cattle%20using%20amelogenin%20gene%20primers.pdf http://psasir.upm.edu.my/id/eprint/6568/ http://www.indianjournals.com/ijor.aspx?target=ijor:jaar&volume=35&issue=1&article=017 |
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| Summary: | To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments of 280 bp (AMELX) and 213 bp (AMELY) for the males. Thus the SE47/48 primer pair is not only reliable for sex differentiation in B. indicus but may also be used to differentiate the B. indicus and B. taurus males. |
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