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Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium

Aims: The use of microalgae as source of natural antioxidants is under explored in Malaysia. Previous studies have shown that microalgae contain minerals, polysaccharides, amino derivatives, carotenoids and phenolic compounds. This study aimed to determine total phenolic and flavonoid compounds and...

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Main Authors: Azim, Nur Husna, Subki, Atiqah, Balia Yusof, Zetty Norhana
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2018
Online Access:http://psasir.upm.edu.my/id/eprint/63944/1/Abiotic%20stresses%20induce%20total%20phenolic%2C%20total%20flavonoid%20and%20antioxidant%20properties%20in%20Malaysian%20indigenous%20microalgae%20and%20cyanobacterium.pdf
http://psasir.upm.edu.my/id/eprint/63944/
http://mjm.usm.my/index.php?r=cms/entry/view&id=51
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spelling my.upm.eprints.639442018-06-08T00:25:32Z http://psasir.upm.edu.my/id/eprint/63944/ Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium Azim, Nur Husna Subki, Atiqah Balia Yusof, Zetty Norhana Aims: The use of microalgae as source of natural antioxidants is under explored in Malaysia. Previous studies have shown that microalgae contain minerals, polysaccharides, amino derivatives, carotenoids and phenolic compounds. This study aimed to determine total phenolic and flavonoid compounds and antioxidant activity when microalgae (Nannochloropsis oculata and Tetraselmis sp.) and cyanobacterium (Anabaena sp.) were subjected to abiotic stresses. Methodology and results: Treatment of sodium chloride (NaCI), sodium hypochlorite (NaOCI) and copper (Cu2+) were given when the cultures reached the exponential phase of growth and were collected at three different time points. Nontreated cultures were used as controls. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau phenol reagent and aluminium chloride colorimetric assays. Antioxidant activities were measured using 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging activity assay. Tetraselmis sp. exhibited the highest phenolic content under copper stress (10.35 ± 0.33 µg GAE/mg extract). Nannochloropsis oculata showed the highest total flavonoid content under copper stress (33.85±3.16 µg QE/mg extract). Anabaena sp. showed the highest radical scavenging activity under NaOCI stress (96.42 ± 0.26%). Conclusion, significance and impact of study: This study showed that total phenolic, flavonoid and antioxidant activities in treated cultures were high compared to non-treated cultures. These microorganisms could be utilized as a source of useful bioactive compounds while exploiting its abundance. Malaysian Society for Microbiology 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63944/1/Abiotic%20stresses%20induce%20total%20phenolic%2C%20total%20flavonoid%20and%20antioxidant%20properties%20in%20Malaysian%20indigenous%20microalgae%20and%20cyanobacterium.pdf Azim, Nur Husna and Subki, Atiqah and Balia Yusof, Zetty Norhana (2018) Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium. Malaysian Journal of Microbiology, 14 (1). pp. 25-33. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Aims: The use of microalgae as source of natural antioxidants is under explored in Malaysia. Previous studies have shown that microalgae contain minerals, polysaccharides, amino derivatives, carotenoids and phenolic compounds. This study aimed to determine total phenolic and flavonoid compounds and antioxidant activity when microalgae (Nannochloropsis oculata and Tetraselmis sp.) and cyanobacterium (Anabaena sp.) were subjected to abiotic stresses. Methodology and results: Treatment of sodium chloride (NaCI), sodium hypochlorite (NaOCI) and copper (Cu2+) were given when the cultures reached the exponential phase of growth and were collected at three different time points. Nontreated cultures were used as controls. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau phenol reagent and aluminium chloride colorimetric assays. Antioxidant activities were measured using 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging activity assay. Tetraselmis sp. exhibited the highest phenolic content under copper stress (10.35 ± 0.33 µg GAE/mg extract). Nannochloropsis oculata showed the highest total flavonoid content under copper stress (33.85±3.16 µg QE/mg extract). Anabaena sp. showed the highest radical scavenging activity under NaOCI stress (96.42 ± 0.26%). Conclusion, significance and impact of study: This study showed that total phenolic, flavonoid and antioxidant activities in treated cultures were high compared to non-treated cultures. These microorganisms could be utilized as a source of useful bioactive compounds while exploiting its abundance.
format Article
author Azim, Nur Husna
Subki, Atiqah
Balia Yusof, Zetty Norhana
spellingShingle Azim, Nur Husna
Subki, Atiqah
Balia Yusof, Zetty Norhana
Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium
author_facet Azim, Nur Husna
Subki, Atiqah
Balia Yusof, Zetty Norhana
author_sort Azim, Nur Husna
title Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium
title_short Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium
title_full Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium
title_fullStr Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium
title_full_unstemmed Abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in Malaysian indigenous microalgae and cyanobacterium
title_sort abiotic stresses induce total phenolic, total flavonoid and antioxidant properties in malaysian indigenous microalgae and cyanobacterium
publisher Malaysian Society for Microbiology
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/63944/1/Abiotic%20stresses%20induce%20total%20phenolic%2C%20total%20flavonoid%20and%20antioxidant%20properties%20in%20Malaysian%20indigenous%20microalgae%20and%20cyanobacterium.pdf
http://psasir.upm.edu.my/id/eprint/63944/
http://mjm.usm.my/index.php?r=cms/entry/view&id=51
_version_ 1643837880743755776
score 13.211869

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