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Risk of Escherichia coli O157:H7 infection linked to the consumption of beef

Escherichia coli O157:H7 is a major food-borne pathogen that has resulted in numerous outbreaks around the world. Widespread distribution of the organism in various ecological niches impedes the control measures. This study aimed to detect and quantify E. coli O157:H7 in beef sold in wet markets...

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Main Authors: Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari, New, Chia Yeung, Anyi, Ubong, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Radu, Son
Format: Article
Language:English
Published: Rynnye Lyan Resources 2017
Online Access:http://psasir.upm.edu.my/id/eprint/63002/1/Risk%20of%20Escherichia%20coli%20O157H7%20infection%20linked%20to%20the%20consumption%20of%20beef.pdf
http://psasir.upm.edu.my/id/eprint/63002/
https://www.myfoodresearch.com/vol-19474issue-3.html
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spelling my.upm.eprints.630022020-05-15T08:07:44Z http://psasir.upm.edu.my/id/eprint/63002/ Risk of Escherichia coli O157:H7 infection linked to the consumption of beef Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari New, Chia Yeung Anyi, Ubong Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Escherichia coli O157:H7 is a major food-borne pathogen that has resulted in numerous outbreaks around the world. Widespread distribution of the organism in various ecological niches impedes the control measures. This study aimed to detect and quantify E. coli O157:H7 in beef sold in wet markets and hypermarkets in Malaysia and to determine the risk of E. coli O157:H7 infection linked to consumption of beef. The rfb O157 and flicH7 primers targeted on somatic antigen (O157) and flagellar antigen (H7) respectively of E. coli O157:H7 was used for the MPN-PCR method. A total of 99 beef samples were collected from local wet markets and hypermarkets. The highest E. coli O157:H7 contamination rate was observed in beef samples collected from wet markets (89.50%), whereas the contamination rate in hyper market A and B were compratively low (35.35 and 20% respectively). However, the microbial load was highest in the beef samples from hypermarket A (1100 MPN/g) while E. coli O157:H7 bacterial load in beef samples from hypermarket B and wet market ranged from 3 to 93 MPN/g and 3 to 240 MPN/g, respectively. Using the Quantitative Microbial Risk Assessment (QMRA) approach the risk was estimated incorporating the findings of the prevalence study and predictions based on home storage, cooking and consumption patterns. Three different exposure pathways were investigated to estimate the risk associated with contaminated beef and Monte Carlo simulation was used to determine the level of uncertainty. The developed model predicated that consumption of contaminated beef can be accountable for 1.83E+06 E. coli O157:H7 cases per year in Malaysia. The reliability of the model, data gaps and further research needs, is discussed. Through continuous improvement Quantitative Microbial Risk Assessment provides valuable insight into controlling and prevention strategies. Rynnye Lyan Resources 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63002/1/Risk%20of%20Escherichia%20coli%20O157H7%20infection%20linked%20to%20the%20consumption%20of%20beef.pdf Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari and New, Chia Yeung and Anyi, Ubong and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2017) Risk of Escherichia coli O157:H7 infection linked to the consumption of beef. Food Research, 1 (3). pp. 67-76. ISSN 2550-2166 https://www.myfoodresearch.com/vol-19474issue-3.html 10.26656/fr.2017.3.011
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Escherichia coli O157:H7 is a major food-borne pathogen that has resulted in numerous outbreaks around the world. Widespread distribution of the organism in various ecological niches impedes the control measures. This study aimed to detect and quantify E. coli O157:H7 in beef sold in wet markets and hypermarkets in Malaysia and to determine the risk of E. coli O157:H7 infection linked to consumption of beef. The rfb O157 and flicH7 primers targeted on somatic antigen (O157) and flagellar antigen (H7) respectively of E. coli O157:H7 was used for the MPN-PCR method. A total of 99 beef samples were collected from local wet markets and hypermarkets. The highest E. coli O157:H7 contamination rate was observed in beef samples collected from wet markets (89.50%), whereas the contamination rate in hyper market A and B were compratively low (35.35 and 20% respectively). However, the microbial load was highest in the beef samples from hypermarket A (1100 MPN/g) while E. coli O157:H7 bacterial load in beef samples from hypermarket B and wet market ranged from 3 to 93 MPN/g and 3 to 240 MPN/g, respectively. Using the Quantitative Microbial Risk Assessment (QMRA) approach the risk was estimated incorporating the findings of the prevalence study and predictions based on home storage, cooking and consumption patterns. Three different exposure pathways were investigated to estimate the risk associated with contaminated beef and Monte Carlo simulation was used to determine the level of uncertainty. The developed model predicated that consumption of contaminated beef can be accountable for 1.83E+06 E. coli O157:H7 cases per year in Malaysia. The reliability of the model, data gaps and further research needs, is discussed. Through continuous improvement Quantitative Microbial Risk Assessment provides valuable insight into controlling and prevention strategies.
format Article
author Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari
New, Chia Yeung
Anyi, Ubong
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
spellingShingle Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari
New, Chia Yeung
Anyi, Ubong
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
author_facet Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari
New, Chia Yeung
Anyi, Ubong
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
author_sort Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari
title Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
title_short Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
title_full Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
title_fullStr Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
title_full_unstemmed Risk of Escherichia coli O157:H7 infection linked to the consumption of beef
title_sort risk of escherichia coli o157:h7 infection linked to the consumption of beef
publisher Rynnye Lyan Resources
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/63002/1/Risk%20of%20Escherichia%20coli%20O157H7%20infection%20linked%20to%20the%20consumption%20of%20beef.pdf
http://psasir.upm.edu.my/id/eprint/63002/
https://www.myfoodresearch.com/vol-19474issue-3.html
_version_ 1669008804665098240
score 13.211869

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