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Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension

PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produ...

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Main Authors: Karim, Mohd Shahrul Nizwanshah, Abdullah, Siti Nor Akmar
Format: Conference or Workshop Item
Language:English
Published: Institute of Plantation Studies, Universiti Putra Malaysia 2017
Online Access:http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf
http://psasir.upm.edu.my/id/eprint/58843/
http://spel2.upm.edu.my/webupm/upload/dokumen/penerbitan/20180102001241ICBAA2017_Poster_Paper_21.pdf
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spelling my.upm.eprints.588432018-02-07T03:40:32Z http://psasir.upm.edu.my/id/eprint/58843/ Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension Karim, Mohd Shahrul Nizwanshah Abdullah, Siti Nor Akmar PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produce full length product with the site-directed mutation. The internal primer serves to introduce nucleotide substitution for site-directed mutagenesis by complementary 3’ ends on the intermediate segments. The full-length product amplied by the flanking primers in the second stage PCR includes the restriction enzyme sites for inserting the product into an expression vector for cloning purposes. Institute of Plantation Studies, Universiti Putra Malaysia 2017 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf Karim, Mohd Shahrul Nizwanshah and Abdullah, Siti Nor Akmar (2017) Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension. In: International Conference on Big Data Applications in Agriculture (ICBAA2017), 5-6 Dec. 2017, Auditorium Putra, TNCPI Building, Universiti Putra Malaysia. (pp. 191-195). http://spel2.upm.edu.my/webupm/upload/dokumen/penerbitan/20180102001241ICBAA2017_Poster_Paper_21.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produce full length product with the site-directed mutation. The internal primer serves to introduce nucleotide substitution for site-directed mutagenesis by complementary 3’ ends on the intermediate segments. The full-length product amplied by the flanking primers in the second stage PCR includes the restriction enzyme sites for inserting the product into an expression vector for cloning purposes.
format Conference or Workshop Item
author Karim, Mohd Shahrul Nizwanshah
Abdullah, Siti Nor Akmar
spellingShingle Karim, Mohd Shahrul Nizwanshah
Abdullah, Siti Nor Akmar
Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
author_facet Karim, Mohd Shahrul Nizwanshah
Abdullah, Siti Nor Akmar
author_sort Karim, Mohd Shahrul Nizwanshah
title Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
title_short Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
title_full Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
title_fullStr Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
title_full_unstemmed Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
title_sort mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (hggt) by pcr-driven overlap extension
publisher Institute of Plantation Studies, Universiti Putra Malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf
http://psasir.upm.edu.my/id/eprint/58843/
http://spel2.upm.edu.my/webupm/upload/dokumen/penerbitan/20180102001241ICBAA2017_Poster_Paper_21.pdf
_version_ 1643836902668763136
score 13.211869

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