Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produ...
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| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Institute of Plantation Studies, Universiti Putra Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf http://psasir.upm.edu.my/id/eprint/58843/ http://spel2.upm.edu.my/webupm/upload/dokumen/penerbitan/20180102001241ICBAA2017_Poster_Paper_21.pdf |
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| Summary: | PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produce full length product with the site-directed mutation. The internal primer serves to introduce nucleotide substitution for site-directed mutagenesis by complementary 3’ ends on the intermediate segments. The full-length product amplied by the flanking primers in the second stage PCR includes the restriction enzyme sites for inserting the product into an expression vector for cloning purposes. |
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