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Application of membrane-based technology for purification of bromelain

About 60% of world’s commercial enzyme products are proteases, giving promising opportunity to derive such enzymes sustainably from waste sources. Bromelain is a crude protease occurring naturally in pineapple, and it possesses properties of benefit for pharmaceutical, medical and food products. The...

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Main Authors: Mohd Nor, Mohd Zuhair, Ramchandran, Lata, Duke, Mikel, Vasiljevic, Todor
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2017
Online Access:http://psasir.upm.edu.my/id/eprint/58366/1/%2845%29.pdf
http://psasir.upm.edu.my/id/eprint/58366/
http://www.ifrj.upm.edu.my/24%20(04)%202017/(45).pdf
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spelling my.upm.eprints.583662018-01-12T05:41:52Z http://psasir.upm.edu.my/id/eprint/58366/ Application of membrane-based technology for purification of bromelain Mohd Nor, Mohd Zuhair Ramchandran, Lata Duke, Mikel Vasiljevic, Todor About 60% of world’s commercial enzyme products are proteases, giving promising opportunity to derive such enzymes sustainably from waste sources. Bromelain is a crude protease occurring naturally in pineapple, and it possesses properties of benefit for pharmaceutical, medical and food products. The production of bromelain involves a purification stage, normally performed by small-scale conventional operations which lead to high operating cost and low product recovery, while being difficult to scale up and produce polluting by-products. Membrane-based technology offers an alternative to produce high quality purified bromelain in a more efficient and sustainable process. This review identified the current state and future needs for utilising membrane processes for sustainable bromelain production at larger scales. It was found that declining membrane flux due to fouling have been reported, but may be effectively overcome with more appropriate (and advanced) membrane types and/or processing conditions. For example, interactions between macromolecules present in the pineapple derived bromelain mixture (particularly polysaccharides) and the membrane may cause performance limiting fouling, but can be overcome by enzymatic pre-treatment. Membrane fouling can be further reduced by the employment of ceramic membrane filters operating at optimised trans-membrane pressure, cross-flow velocity, feed pH and temperature. Two-stage ultrafiltration together with diafiltration or gas sparging was suggested as a means to reduce fouling and improve enzyme purity. Despite these promising technical findings, the review identified the need for a valid economic assessment to properly guide further work towards purifying bromelain from pineapple waste for sustainable production of commercial proteases. Faculty of Food Science and Technology, Universiti Putra Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58366/1/%2845%29.pdf Mohd Nor, Mohd Zuhair and Ramchandran, Lata and Duke, Mikel and Vasiljevic, Todor (2017) Application of membrane-based technology for purification of bromelain. International Food Research Journal, 24 (4). pp. 1685-1696. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/24%20(04)%202017/(45).pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description About 60% of world’s commercial enzyme products are proteases, giving promising opportunity to derive such enzymes sustainably from waste sources. Bromelain is a crude protease occurring naturally in pineapple, and it possesses properties of benefit for pharmaceutical, medical and food products. The production of bromelain involves a purification stage, normally performed by small-scale conventional operations which lead to high operating cost and low product recovery, while being difficult to scale up and produce polluting by-products. Membrane-based technology offers an alternative to produce high quality purified bromelain in a more efficient and sustainable process. This review identified the current state and future needs for utilising membrane processes for sustainable bromelain production at larger scales. It was found that declining membrane flux due to fouling have been reported, but may be effectively overcome with more appropriate (and advanced) membrane types and/or processing conditions. For example, interactions between macromolecules present in the pineapple derived bromelain mixture (particularly polysaccharides) and the membrane may cause performance limiting fouling, but can be overcome by enzymatic pre-treatment. Membrane fouling can be further reduced by the employment of ceramic membrane filters operating at optimised trans-membrane pressure, cross-flow velocity, feed pH and temperature. Two-stage ultrafiltration together with diafiltration or gas sparging was suggested as a means to reduce fouling and improve enzyme purity. Despite these promising technical findings, the review identified the need for a valid economic assessment to properly guide further work towards purifying bromelain from pineapple waste for sustainable production of commercial proteases.
format Article
author Mohd Nor, Mohd Zuhair
Ramchandran, Lata
Duke, Mikel
Vasiljevic, Todor
spellingShingle Mohd Nor, Mohd Zuhair
Ramchandran, Lata
Duke, Mikel
Vasiljevic, Todor
Application of membrane-based technology for purification of bromelain
author_facet Mohd Nor, Mohd Zuhair
Ramchandran, Lata
Duke, Mikel
Vasiljevic, Todor
author_sort Mohd Nor, Mohd Zuhair
title Application of membrane-based technology for purification of bromelain
title_short Application of membrane-based technology for purification of bromelain
title_full Application of membrane-based technology for purification of bromelain
title_fullStr Application of membrane-based technology for purification of bromelain
title_full_unstemmed Application of membrane-based technology for purification of bromelain
title_sort application of membrane-based technology for purification of bromelain
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/58366/1/%2845%29.pdf
http://psasir.upm.edu.my/id/eprint/58366/
http://www.ifrj.upm.edu.my/24%20(04)%202017/(45).pdf
_version_ 1643836762445840384
score 13.211869

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