Cover Image

Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey

Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α...

Full description

Saved in:
Bibliographic Details
Main Authors: Balasubramaniam, Vimala, June, Chelyn Lee, Mohd Noh, Mohd Fairunizal, Ahmad, Syahida, Brownlee, Iain A., Ismail, Amin
Format: Article
Language:English
Published: Springer 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54996/1/Alpha-amylase%2C%20antioxidant%2C%20and%20anti-inflammatory%20.pdf
http://psasir.upm.edu.my/id/eprint/54996/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.54996
record_format eprints
spelling my.upm.eprints.549962018-07-12T09:44:09Z http://psasir.upm.edu.my/id/eprint/54996/ Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey Balasubramaniam, Vimala June, Chelyn Lee Mohd Noh, Mohd Fairunizal Ahmad, Syahida Brownlee, Iain A. Ismail, Amin Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α-Amylase activity was assessed by dinitrosalicylic acid (DNS) assay, while the antioxidant property was determined by oxygen radical absorbance capacity (ORAC) analysis. The anti-inflammatory effects of the seaweed samples were evaluated by nitric oxide (NO), interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) activities on the interferon-gamma/lipopolysaccharide (IFN-γ/LPS)-stimulated murine macrophage cell line (RAW 264.7) using Griess reaction and immunoassays, respectively. At 10 mg mL−1, E. denticulatum ethanol extract and three fractions inhibited α-amylase activities at variable levels. The highest (67 %) inhibition of α-amylase enzyme was by the ethanol crude extract. The three fractions showed inhibition with a mean of 42 %. Crude ethanol extracts also exhibited higher antioxidant capacity (36,400 ± 23.5 mol Trolox equivalent (TE) (100 g)−1) when compared to the fractions. Crude extract and fractions (1–100 μg mL−1), also exhibited anti-inflammatory activity without showing any cytotoxic effect to RAW 264.7 cells. The present study suggests that E. denticulatum has the potential to be a promising source of effective functional metabolite. An extensive research on the edible varieties would contribute to a better understanding of their importance as functional food. Springer 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54996/1/Alpha-amylase%2C%20antioxidant%2C%20and%20anti-inflammatory%20.pdf Balasubramaniam, Vimala and June, Chelyn Lee and Mohd Noh, Mohd Fairunizal and Ahmad, Syahida and Brownlee, Iain A. and Ismail, Amin (2016) Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey. Journal of Applied Phycology, 28. pp. 1965-1974. ISSN 0921-8971; ESSN: 1573-5176 10.1007/s10811-015-0690-6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α-Amylase activity was assessed by dinitrosalicylic acid (DNS) assay, while the antioxidant property was determined by oxygen radical absorbance capacity (ORAC) analysis. The anti-inflammatory effects of the seaweed samples were evaluated by nitric oxide (NO), interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) activities on the interferon-gamma/lipopolysaccharide (IFN-γ/LPS)-stimulated murine macrophage cell line (RAW 264.7) using Griess reaction and immunoassays, respectively. At 10 mg mL−1, E. denticulatum ethanol extract and three fractions inhibited α-amylase activities at variable levels. The highest (67 %) inhibition of α-amylase enzyme was by the ethanol crude extract. The three fractions showed inhibition with a mean of 42 %. Crude ethanol extracts also exhibited higher antioxidant capacity (36,400 ± 23.5 mol Trolox equivalent (TE) (100 g)−1) when compared to the fractions. Crude extract and fractions (1–100 μg mL−1), also exhibited anti-inflammatory activity without showing any cytotoxic effect to RAW 264.7 cells. The present study suggests that E. denticulatum has the potential to be a promising source of effective functional metabolite. An extensive research on the edible varieties would contribute to a better understanding of their importance as functional food.
format Article
author Balasubramaniam, Vimala
June, Chelyn Lee
Mohd Noh, Mohd Fairunizal
Ahmad, Syahida
Brownlee, Iain A.
Ismail, Amin
spellingShingle Balasubramaniam, Vimala
June, Chelyn Lee
Mohd Noh, Mohd Fairunizal
Ahmad, Syahida
Brownlee, Iain A.
Ismail, Amin
Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
author_facet Balasubramaniam, Vimala
June, Chelyn Lee
Mohd Noh, Mohd Fairunizal
Ahmad, Syahida
Brownlee, Iain A.
Ismail, Amin
author_sort Balasubramaniam, Vimala
title Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
title_short Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
title_full Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
title_fullStr Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
title_full_unstemmed Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
title_sort alpha-amylase, antioxidant, and anti-inflammatory activities of eucheuma denticulatum (n.l. burman) f.s. collins and hervey
publisher Springer
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54996/1/Alpha-amylase%2C%20antioxidant%2C%20and%20anti-inflammatory%20.pdf
http://psasir.upm.edu.my/id/eprint/54996/
_version_ 1643835768155668480
score 13.211869

Similar Items