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Identification and characterization of genes involved in the fruit color development of european plum

European plum fruit (Prunus domestica) are normally blue-black to dark purple. However, some genotypes remain green/yellow after ripening. We hypothesized that in such genotypes anthocyanin biosynthesis is genetically disturbed. To examine this hypothesis, six european plum genotypes with diverse fr...

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Main Authors: Selvaraj, Dineshkumar, Sherif, Sherif, Pak Dek, Mohd Sabri, Paliyath, Gopinadhan, El-Sharkawy, Islam, Subramanian, Jayasankar
Format: Article
Language:English
Published: American Society for Horticultural Science 2016
Online Access:http://psasir.upm.edu.my/id/eprint/54475/1/Identification%20and%20characterization%20of%20genes%20involved%20in%20the%20fruit%20color%20development%20of%20european%20plum.pdf
http://psasir.upm.edu.my/id/eprint/54475/
http://journal.ashspublications.org/content/141/5/467.short
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spelling my.upm.eprints.544752018-03-20T01:34:53Z http://psasir.upm.edu.my/id/eprint/54475/ Identification and characterization of genes involved in the fruit color development of european plum Selvaraj, Dineshkumar Sherif, Sherif Pak Dek, Mohd Sabri Paliyath, Gopinadhan El-Sharkawy, Islam Subramanian, Jayasankar European plum fruit (Prunus domestica) are normally blue-black to dark purple. However, some genotypes remain green/yellow after ripening. We hypothesized that in such genotypes anthocyanin biosynthesis is genetically disturbed. To examine this hypothesis, six european plum genotypes with diverse fruit colors were investigated for the expression pattern of several anthocyanin biosynthetic genes (ABGs)—e.g., phenylalanine ammonia-lyase, chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), anthocyanin synthase (ANS), and UDP-glucose:flavonoid 3-O-glucosyltransferase 1 and 2 (UFGT 1 and 2). Expression profiles indicated that ABGs, especially Pd-CHS and UFGT 2, were significantly downregulated in the green/yellow fruit compared with the dark-purple fruit. Furthermore, the quantification of total polyphenols and individual flavonoid compounds showed substantial differences between the off-colored and the purple genotype. To further examine the contribution of each of the ABGs in color development, the open reading frame (ORP) of Pd-CHS, Pd-DFR, Pd-ANS, and Pd-UFGT 2 was ectopically expressed in tobacco (Nicotiana tabacum). The characterization of transgenic plants showed that the petals of plants expressing Pd-CHS were darker in color and had higher anthocyanin content than control or even other transgenic types, suggesting the significant contribution of CHS in determining anthocyanin production levels and hence fruit coloration. The results of this study provides better understanding of color development in european plum, which can be rewarding in developing european plum cultivars with desired colors through classical or modern breeding tools. American Society for Horticultural Science 2016-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54475/1/Identification%20and%20characterization%20of%20genes%20involved%20in%20the%20fruit%20color%20development%20of%20european%20plum.pdf Selvaraj, Dineshkumar and Sherif, Sherif and Pak Dek, Mohd Sabri and Paliyath, Gopinadhan and El-Sharkawy, Islam and Subramanian, Jayasankar (2016) Identification and characterization of genes involved in the fruit color development of european plum. Journal of the American Society for Horticultural Science, 141 (5). pp. 467-474. ISSN 0003-1062; ESSN: 2327-9788 http://journal.ashspublications.org/content/141/5/467.short 10.21273/JASHS03617-16
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description European plum fruit (Prunus domestica) are normally blue-black to dark purple. However, some genotypes remain green/yellow after ripening. We hypothesized that in such genotypes anthocyanin biosynthesis is genetically disturbed. To examine this hypothesis, six european plum genotypes with diverse fruit colors were investigated for the expression pattern of several anthocyanin biosynthetic genes (ABGs)—e.g., phenylalanine ammonia-lyase, chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), anthocyanin synthase (ANS), and UDP-glucose:flavonoid 3-O-glucosyltransferase 1 and 2 (UFGT 1 and 2). Expression profiles indicated that ABGs, especially Pd-CHS and UFGT 2, were significantly downregulated in the green/yellow fruit compared with the dark-purple fruit. Furthermore, the quantification of total polyphenols and individual flavonoid compounds showed substantial differences between the off-colored and the purple genotype. To further examine the contribution of each of the ABGs in color development, the open reading frame (ORP) of Pd-CHS, Pd-DFR, Pd-ANS, and Pd-UFGT 2 was ectopically expressed in tobacco (Nicotiana tabacum). The characterization of transgenic plants showed that the petals of plants expressing Pd-CHS were darker in color and had higher anthocyanin content than control or even other transgenic types, suggesting the significant contribution of CHS in determining anthocyanin production levels and hence fruit coloration. The results of this study provides better understanding of color development in european plum, which can be rewarding in developing european plum cultivars with desired colors through classical or modern breeding tools.
format Article
author Selvaraj, Dineshkumar
Sherif, Sherif
Pak Dek, Mohd Sabri
Paliyath, Gopinadhan
El-Sharkawy, Islam
Subramanian, Jayasankar
spellingShingle Selvaraj, Dineshkumar
Sherif, Sherif
Pak Dek, Mohd Sabri
Paliyath, Gopinadhan
El-Sharkawy, Islam
Subramanian, Jayasankar
Identification and characterization of genes involved in the fruit color development of european plum
author_facet Selvaraj, Dineshkumar
Sherif, Sherif
Pak Dek, Mohd Sabri
Paliyath, Gopinadhan
El-Sharkawy, Islam
Subramanian, Jayasankar
author_sort Selvaraj, Dineshkumar
title Identification and characterization of genes involved in the fruit color development of european plum
title_short Identification and characterization of genes involved in the fruit color development of european plum
title_full Identification and characterization of genes involved in the fruit color development of european plum
title_fullStr Identification and characterization of genes involved in the fruit color development of european plum
title_full_unstemmed Identification and characterization of genes involved in the fruit color development of european plum
title_sort identification and characterization of genes involved in the fruit color development of european plum
publisher American Society for Horticultural Science
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54475/1/Identification%20and%20characterization%20of%20genes%20involved%20in%20the%20fruit%20color%20development%20of%20european%20plum.pdf
http://psasir.upm.edu.my/id/eprint/54475/
http://journal.ashspublications.org/content/141/5/467.short
_version_ 1643835644189868032
score 13.211869

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