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Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)

This report shows the partitioning and purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) by solid-state fermentation (SSF). In the present analysis, some of the important parameters such as PEG concentration, PEG molecular mass, salt concentration and buffer conce...

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Main Authors: Alhelli, Amaal M., Abdul Manap, Mohd Yazid, Mohammed, Abdulkarim Sabo, Mirhosseini, Hamed, Suliman, Eilaf, Shad, Zahra, Mohammed, Nameer Khairullah, Meor Hussin, Anis Shobirin
Format: Article
Published: Elsevier BV 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53207/
http://www.elsevier.com/locate/issn/15700232
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spelling my.upm.eprints.532072017-10-27T03:27:44Z http://psasir.upm.edu.my/id/eprint/53207/ Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) Alhelli, Amaal M. Abdul Manap, Mohd Yazid Mohammed, Abdulkarim Sabo Mirhosseini, Hamed Suliman, Eilaf Shad, Zahra Mohammed, Nameer Khairullah Meor Hussin, Anis Shobirin This report shows the partitioning and purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) by solid-state fermentation (SSF). In the present analysis, some of the important parameters such as PEG concentration, PEG molecular mass, salt concentration and buffer concentration were optimised through the response surface methodology (RSM). The optimum aqueous two-phase systems (ATPS) environment consisted of 13.8% (w/w) phosphate buffer, 9.2% (w/w) PEG-3000 and 3.3% (w/w) NaCl at 25°C. The RSM approach was proved to be the most suitable methodology for the recovery of desired enzymes. In this method, the enzyme partitioned into the top phase of the PEG-buffer-NaCl ATPS. Under this experimental environment, the purification factor was found to be 33.9, the partition coefficient was 4.0 and the yield was found to be 84.0% of lipase. Moreover, the experimental and predicted results were in considerable agreement, which established the reliability and validity of the proposed model. The ATPS methodology is proven to be effective for the primary recovery of lipase at a low cost with a large loading capacity and possibility of linear scale up. In addition to using the existing methodologies for improving enzyme production, the use of statistical optimisation of the constituents of phases through RSM continues to be the basic and practical method. Elsevier BV 2016 Article PeerReviewed Alhelli, Amaal M. and Abdul Manap, Mohd Yazid and Mohammed, Abdulkarim Sabo and Mirhosseini, Hamed and Suliman, Eilaf and Shad, Zahra and Mohammed, Nameer Khairullah and Meor Hussin, Anis Shobirin (2016) Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031). Journal of Chromatography B, 1039. pp. 66-73. ISSN 1570-0232 http://www.elsevier.com/locate/issn/15700232 10.1016/j.jchromb.2016.10.037
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description This report shows the partitioning and purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) by solid-state fermentation (SSF). In the present analysis, some of the important parameters such as PEG concentration, PEG molecular mass, salt concentration and buffer concentration were optimised through the response surface methodology (RSM). The optimum aqueous two-phase systems (ATPS) environment consisted of 13.8% (w/w) phosphate buffer, 9.2% (w/w) PEG-3000 and 3.3% (w/w) NaCl at 25°C. The RSM approach was proved to be the most suitable methodology for the recovery of desired enzymes. In this method, the enzyme partitioned into the top phase of the PEG-buffer-NaCl ATPS. Under this experimental environment, the purification factor was found to be 33.9, the partition coefficient was 4.0 and the yield was found to be 84.0% of lipase. Moreover, the experimental and predicted results were in considerable agreement, which established the reliability and validity of the proposed model. The ATPS methodology is proven to be effective for the primary recovery of lipase at a low cost with a large loading capacity and possibility of linear scale up. In addition to using the existing methodologies for improving enzyme production, the use of statistical optimisation of the constituents of phases through RSM continues to be the basic and practical method.
format Article
author Alhelli, Amaal M.
Abdul Manap, Mohd Yazid
Mohammed, Abdulkarim Sabo
Mirhosseini, Hamed
Suliman, Eilaf
Shad, Zahra
Mohammed, Nameer Khairullah
Meor Hussin, Anis Shobirin
spellingShingle Alhelli, Amaal M.
Abdul Manap, Mohd Yazid
Mohammed, Abdulkarim Sabo
Mirhosseini, Hamed
Suliman, Eilaf
Shad, Zahra
Mohammed, Nameer Khairullah
Meor Hussin, Anis Shobirin
Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)
author_facet Alhelli, Amaal M.
Abdul Manap, Mohd Yazid
Mohammed, Abdulkarim Sabo
Mirhosseini, Hamed
Suliman, Eilaf
Shad, Zahra
Mohammed, Nameer Khairullah
Meor Hussin, Anis Shobirin
author_sort Alhelli, Amaal M.
title Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)
title_short Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)
title_full Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)
title_fullStr Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)
title_full_unstemmed Use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)
title_sort use of response surface methodology for partitioning, one-step purification, of alkaline extracellular lipase from penicillium candidum (pca 1/tt031)
publisher Elsevier BV
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53207/
http://www.elsevier.com/locate/issn/15700232
_version_ 1643835340922814464
score 13.211869

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