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Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.

Regulations for the use and labeling of genetically modified organism (GMO) products and derived ingredients are being implemented worldwide, that demands reliable and accurate methods to detect GMO in raw materials and food products. In this study, polymerase chain reaction (PCR) method was establi...

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Main Authors: Abdullah, Tosiah, Radu, Son, Hassan, Zaiton, Hashim, Jamal Khair
Format: Article
Language:English
English
Published: Elsevier 2006
Online Access:http://psasir.upm.edu.my/id/eprint/4709/1/Detection%20of%20genetically%20modified%20soy%20in%20processed%20foods%20sold%20commercially%20in%20Malaysia%20by%20PCR.pdf
http://psasir.upm.edu.my/id/eprint/4709/
http://dx.doi.org/10.1016/j.foodchem.2005.07.035
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spelling my.upm.eprints.47092015-12-09T02:56:42Z http://psasir.upm.edu.my/id/eprint/4709/ Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method. Abdullah, Tosiah Radu, Son Hassan, Zaiton Hashim, Jamal Khair Regulations for the use and labeling of genetically modified organism (GMO) products and derived ingredients are being implemented worldwide, that demands reliable and accurate methods to detect GMO in raw materials and food products. In this study, polymerase chain reaction (PCR) method was established for monitoring products derived from GMO that are sold in the markets in Malaysia, which specifically amplify the 35S promoter, nos (nopaline synthase-terminator), EPSPS (5-enolpyruvylshikimate-3- phosphate synthase) and RRS (CTP/CP4EPSPS). Using this method, we investigated the incidence of genetically modified soy(GM-soy) and specifically the presence of roundup ready soy (RRS). All the soybean samples were evidenced by presence of the lectin gene. Out of 85 samples examined, the 18 positive GM samples were raw bean (9), tofu (8) and tempe (1) (a traditional Malayfood). The results demonstrate for the first time the presence of GM-soy in Malaysian food products, reinforcing the need for the development of accurate quantitative methods for routine analyses. Elsevier 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/4709/1/Detection%20of%20genetically%20modified%20soy%20in%20processed%20foods%20sold%20commercially%20in%20Malaysia%20by%20PCR.pdf Abdullah, Tosiah and Radu, Son and Hassan, Zaiton and Hashim, Jamal Khair (2006) Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method. Food Chemistry, 98. pp. 575-579. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2005.07.035 10.1016/j.foodchem.2005.07.035 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Regulations for the use and labeling of genetically modified organism (GMO) products and derived ingredients are being implemented worldwide, that demands reliable and accurate methods to detect GMO in raw materials and food products. In this study, polymerase chain reaction (PCR) method was established for monitoring products derived from GMO that are sold in the markets in Malaysia, which specifically amplify the 35S promoter, nos (nopaline synthase-terminator), EPSPS (5-enolpyruvylshikimate-3- phosphate synthase) and RRS (CTP/CP4EPSPS). Using this method, we investigated the incidence of genetically modified soy(GM-soy) and specifically the presence of roundup ready soy (RRS). All the soybean samples were evidenced by presence of the lectin gene. Out of 85 samples examined, the 18 positive GM samples were raw bean (9), tofu (8) and tempe (1) (a traditional Malayfood). The results demonstrate for the first time the presence of GM-soy in Malaysian food products, reinforcing the need for the development of accurate quantitative methods for routine analyses.
format Article
author Abdullah, Tosiah
Radu, Son
Hassan, Zaiton
Hashim, Jamal Khair
spellingShingle Abdullah, Tosiah
Radu, Son
Hassan, Zaiton
Hashim, Jamal Khair
Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
author_facet Abdullah, Tosiah
Radu, Son
Hassan, Zaiton
Hashim, Jamal Khair
author_sort Abdullah, Tosiah
title Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
title_short Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
title_full Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
title_fullStr Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
title_full_unstemmed Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method.
title_sort detection of genetically modified soy in processed foods sold commercially in malaysia by pcr-based method.
publisher Elsevier
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/4709/1/Detection%20of%20genetically%20modified%20soy%20in%20processed%20foods%20sold%20commercially%20in%20Malaysia%20by%20PCR.pdf
http://psasir.upm.edu.my/id/eprint/4709/
http://dx.doi.org/10.1016/j.foodchem.2005.07.035
_version_ 1643822979350528000
score 13.211869

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