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Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage

Among organic acids, citric acid and their salts are currently the most studied as a supplement in aquafeeds to potentially improve growth and nutrient utilization in fish. The results have been generally beneficial but no studies have been performed on tilapia. A 50 day experiment was conducted on...

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Main Authors: Romano, Nicholas Paul, Simon, Watson, Ebrahimi, Mahdi, Fadel, Abdalbast H. I., Chong, Chou Min, Kamarudin, Mohd Salleh
Format: Article
Language:English
Published: Elsevier 2016
Online Access:http://psasir.upm.edu.my/id/eprint/43277/1/Dietary%20sodium%20citrate%20improved%20oxidative%20stability%20in%20red%20hybrid%20tilapia%20%28Oreochromis%20sp.%29%20but%20reduced%20growth%2C%20health%20status%2C%20intestinal%20short%20chain%20fatty%20acids%20and%20induced%20liver%20damage.pdf
http://psasir.upm.edu.my/id/eprint/43277/
http://www.sciencedirect.com/science/article/pii/S0044848616301235
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spelling my.upm.eprints.432772016-05-18T04:36:26Z http://psasir.upm.edu.my/id/eprint/43277/ Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage Romano, Nicholas Paul Simon, Watson Ebrahimi, Mahdi Fadel, Abdalbast H. I. Chong, Chou Min Kamarudin, Mohd Salleh Among organic acids, citric acid and their salts are currently the most studied as a supplement in aquafeeds to potentially improve growth and nutrient utilization in fish. The results have been generally beneficial but no studies have been performed on tilapia. A 50 day experiment was conducted on the effects of dietary sodium citrate at 0, 1, 2 and 4% on the growth, feeding efficiency, body indices, muscle proximate composition, muscle lipid peroxidation, some plasma and blood parameters, intestinal short chain fatty acids (SCFA), and liver histopathology of red hybrid tilapia (Oreochromis sp.). Triplicate groups of 60 tilapia fingerlings (initial weight of 1.86 ± 0.01) were in each treatment. Results showed that, while not significant, increasing dietary sodium citrate reduced tilapia growth (p > 0.05). However, muscle crude protein (r2 = 0.931), lipid (r2 = 0.962), and ash (r2 = 0.834) significantly decreased at increasing dietary sodium citrate levels (p < 0.05). Plasma ALT significantly increased (p < 0.05; r2 = 0.357) with increasing dietary sodium citrate treatments along with histopathological liver damage that included hemorrhages, necrosis and inflammatory responses. Many of the cell differential counts were significantly (p < 0.05) altered by increasing dietary sodium citrate levels. Among the intestinal SCFA in tilapia, acetic acid was the highest, followed by propionic and butyric acid, and these all significantly decreased (p < 0.05) with increasing dietary sodium citrate. In all dietary sodium citrate treatments, muscle lipid peroxidation was significantly less (p < 0.05; r2 = 0.211) indicating increased oxidative stability. While dietary sodium citrate was toxic to tilapia at the levels used, and is not recommended as a supplement, the decreased lipid peroxidation warrants further investigation with other species. Such research may have important implications for filet quality over prolonged storage. Statement of relevance: Sodium citrate reduced growth but may increase shelf-life. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43277/1/Dietary%20sodium%20citrate%20improved%20oxidative%20stability%20in%20red%20hybrid%20tilapia%20%28Oreochromis%20sp.%29%20but%20reduced%20growth%2C%20health%20status%2C%20intestinal%20short%20chain%20fatty%20acids%20and%20induced%20liver%20damage.pdf Romano, Nicholas Paul and Simon, Watson and Ebrahimi, Mahdi and Fadel, Abdalbast H. I. and Chong, Chou Min and Kamarudin, Mohd Salleh (2016) Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage. Aquaculture, 458. pp. 170-176. ISSN 0044-8486; ESSN: 1873-5622 http://www.sciencedirect.com/science/article/pii/S0044848616301235 10.1016/j.aquaculture.2016.03.014
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Among organic acids, citric acid and their salts are currently the most studied as a supplement in aquafeeds to potentially improve growth and nutrient utilization in fish. The results have been generally beneficial but no studies have been performed on tilapia. A 50 day experiment was conducted on the effects of dietary sodium citrate at 0, 1, 2 and 4% on the growth, feeding efficiency, body indices, muscle proximate composition, muscle lipid peroxidation, some plasma and blood parameters, intestinal short chain fatty acids (SCFA), and liver histopathology of red hybrid tilapia (Oreochromis sp.). Triplicate groups of 60 tilapia fingerlings (initial weight of 1.86 ± 0.01) were in each treatment. Results showed that, while not significant, increasing dietary sodium citrate reduced tilapia growth (p > 0.05). However, muscle crude protein (r2 = 0.931), lipid (r2 = 0.962), and ash (r2 = 0.834) significantly decreased at increasing dietary sodium citrate levels (p < 0.05). Plasma ALT significantly increased (p < 0.05; r2 = 0.357) with increasing dietary sodium citrate treatments along with histopathological liver damage that included hemorrhages, necrosis and inflammatory responses. Many of the cell differential counts were significantly (p < 0.05) altered by increasing dietary sodium citrate levels. Among the intestinal SCFA in tilapia, acetic acid was the highest, followed by propionic and butyric acid, and these all significantly decreased (p < 0.05) with increasing dietary sodium citrate. In all dietary sodium citrate treatments, muscle lipid peroxidation was significantly less (p < 0.05; r2 = 0.211) indicating increased oxidative stability. While dietary sodium citrate was toxic to tilapia at the levels used, and is not recommended as a supplement, the decreased lipid peroxidation warrants further investigation with other species. Such research may have important implications for filet quality over prolonged storage. Statement of relevance: Sodium citrate reduced growth but may increase shelf-life.
format Article
author Romano, Nicholas Paul
Simon, Watson
Ebrahimi, Mahdi
Fadel, Abdalbast H. I.
Chong, Chou Min
Kamarudin, Mohd Salleh
spellingShingle Romano, Nicholas Paul
Simon, Watson
Ebrahimi, Mahdi
Fadel, Abdalbast H. I.
Chong, Chou Min
Kamarudin, Mohd Salleh
Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
author_facet Romano, Nicholas Paul
Simon, Watson
Ebrahimi, Mahdi
Fadel, Abdalbast H. I.
Chong, Chou Min
Kamarudin, Mohd Salleh
author_sort Romano, Nicholas Paul
title Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
title_short Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
title_full Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
title_fullStr Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
title_full_unstemmed Dietary sodium citrate improved oxidative stability in red hybrid tilapia (Oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
title_sort dietary sodium citrate improved oxidative stability in red hybrid tilapia (oreochromis sp.) but reduced growth, health status, intestinal short chain fatty acids and induced liver damage
publisher Elsevier
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/43277/1/Dietary%20sodium%20citrate%20improved%20oxidative%20stability%20in%20red%20hybrid%20tilapia%20%28Oreochromis%20sp.%29%20but%20reduced%20growth%2C%20health%20status%2C%20intestinal%20short%20chain%20fatty%20acids%20and%20induced%20liver%20damage.pdf
http://psasir.upm.edu.my/id/eprint/43277/
http://www.sciencedirect.com/science/article/pii/S0044848616301235
_version_ 1643833519292547072
score 13.211869

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