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Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology

This study aimed to optimise potential extraction conditions using response surface methodology (RSM) for yielding maximum levels of total phenolic content (TPC) and 2,2-diphenyl-1-picryhydrazyl radical (DPPH•) scavenging capacity of henna (Lawsonia inermis) stems. The ranges for selected independen...

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Main Authors: Tan, Mun Chieng, Tan, Chin Ping, Khoo, Hock Eng, Ho, Chun Wai
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2014
Online Access:http://psasir.upm.edu.my/id/eprint/40771/1/50%20IFRJ%2021%20%2802%29%202014%20Tan%20526.pdf
http://psasir.upm.edu.my/id/eprint/40771/
http://www.ifrj.upm.edu.my/21%20%2802%29%202014/50%20IFRJ%2021%20%2802%29%202014%20Tan%20526.pdf
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spelling my.upm.eprints.407712015-10-08T07:59:31Z http://psasir.upm.edu.my/id/eprint/40771/ Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology Tan, Mun Chieng Tan, Chin Ping Khoo, Hock Eng Ho, Chun Wai This study aimed to optimise potential extraction conditions using response surface methodology (RSM) for yielding maximum levels of total phenolic content (TPC) and 2,2-diphenyl-1-picryhydrazyl radical (DPPH•) scavenging capacity of henna (Lawsonia inermis) stems. The ranges for selected independent variables, namely acetone concentration (20-90%, v/v), extraction time (10-90 min) and extraction temperature (25-45°C) were identified by screening tests. Optimum conditions obtained for extraction of TPC were 47.0% acetone, extraction time of 47.6 min and extraction temperature of 37.3°C. The result also showed that 75.8% acetone, extraction time of 26.2 min and extraction temperature of 41°C yielded the highest DPPH• scavenging capacity. The optimized extraction conditions have resulted in TPC and DPPH• scavenging capacity of 5232.4 mg GAE/100 g DW and 6085.7 g TE/100 g DW, respectively which similar to the predicted values. Therefore RSM has successfully optimized the extraction conditions for TPC and radical scavenging capacity of henna stems. Faculty of Food Science and Technology, Universiti Putra Malaysia 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40771/1/50%20IFRJ%2021%20%2802%29%202014%20Tan%20526.pdf Tan, Mun Chieng and Tan, Chin Ping and Khoo, Hock Eng and Ho, Chun Wai (2014) Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology. International Food Research Journal, 21 (2). pp. 789-794. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/21%20%2802%29%202014/50%20IFRJ%2021%20%2802%29%202014%20Tan%20526.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study aimed to optimise potential extraction conditions using response surface methodology (RSM) for yielding maximum levels of total phenolic content (TPC) and 2,2-diphenyl-1-picryhydrazyl radical (DPPH•) scavenging capacity of henna (Lawsonia inermis) stems. The ranges for selected independent variables, namely acetone concentration (20-90%, v/v), extraction time (10-90 min) and extraction temperature (25-45°C) were identified by screening tests. Optimum conditions obtained for extraction of TPC were 47.0% acetone, extraction time of 47.6 min and extraction temperature of 37.3°C. The result also showed that 75.8% acetone, extraction time of 26.2 min and extraction temperature of 41°C yielded the highest DPPH• scavenging capacity. The optimized extraction conditions have resulted in TPC and DPPH• scavenging capacity of 5232.4 mg GAE/100 g DW and 6085.7 g TE/100 g DW, respectively which similar to the predicted values. Therefore RSM has successfully optimized the extraction conditions for TPC and radical scavenging capacity of henna stems.
format Article
author Tan, Mun Chieng
Tan, Chin Ping
Khoo, Hock Eng
Ho, Chun Wai
spellingShingle Tan, Mun Chieng
Tan, Chin Ping
Khoo, Hock Eng
Ho, Chun Wai
Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology
author_facet Tan, Mun Chieng
Tan, Chin Ping
Khoo, Hock Eng
Ho, Chun Wai
author_sort Tan, Mun Chieng
title Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology
title_short Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology
title_full Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology
title_fullStr Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology
title_full_unstemmed Optimization for extraction on total phenolic contentand radical scavenging capacity of henna (Lawsonia inermis) stems using response surface methodology
title_sort optimization for extraction on total phenolic contentand radical scavenging capacity of henna (lawsonia inermis) stems using response surface methodology
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/40771/1/50%20IFRJ%2021%20%2802%29%202014%20Tan%20526.pdf
http://psasir.upm.edu.my/id/eprint/40771/
http://www.ifrj.upm.edu.my/21%20%2802%29%202014/50%20IFRJ%2021%20%2802%29%202014%20Tan%20526.pdf
_version_ 1643832810131161088
score 13.211869

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