Staff View: Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia

Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia

Peanuts are widely consumed in many local dishes in southeast Asian countries, especially in Malaysia which is one of the major peanut-importing countries in this region. Therefore, Aspergillus spp. and aflatoxin contamination in peanuts during storage are becoming major concerns due to the tropical...

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Main Authors:	Mahror, Norlia, Selamat, Jinap, Mahmud @ Ab Rashid, Nor Khaizura, Radu, Son, Chin, Cheow Keat, Samsudin, Nik Iskandar Putra, Abdul Halim, Farawahida
Format:	Article
Language:	English
Published:	MDPI 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/38280/1/38280.pdf http://psasir.upm.edu.my/id/eprint/38280/ https://www.mdpi.com/2072-6651/11/9/501
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spelling	my.upm.eprints.382802020-05-04T16:11:35Z http://psasir.upm.edu.my/id/eprint/38280/ Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia Mahror, Norlia Selamat, Jinap Mahmud @ Ab Rashid, Nor Khaizura Radu, Son Chin, Cheow Keat Samsudin, Nik Iskandar Putra Abdul Halim, Farawahida Peanuts are widely consumed in many local dishes in southeast Asian countries, especially in Malaysia which is one of the major peanut-importing countries in this region. Therefore, Aspergillus spp. and aflatoxin contamination in peanuts during storage are becoming major concerns due to the tropical weather in this region that favours the growth of aflatoxigenic fungi. The present study thus aimed to molecularly identify and characterise the Aspergillus section Flavi isolated from imported peanuts in Malaysia. The internal transcribed spacer (ITS) and β-tubulin sequences were used to confirm the species and determine the phylogenetic relationship among the isolates, while aflatoxin biosynthesis genes (aflR, aflP (omtA), aflD (nor-1), aflM (ver-1), and pksA) were targeted in a multiplex PCR to determine the toxigenic potential. A total of 76 and one isolates were confirmed as A. flavus and A. tamarii, respectively. The Maximum Likelihood (ML) phylogenetic tree resolved the species into two different clades in which all A. flavus (both aflatoxigenic and non-aflatoxigenic) were grouped in the same clade and A. tamarii was grouped in a different clade. The aflatoxin biosynthesis genes were detected in all aflatoxigenic A. flavus while the non-aflatoxigenic A. flavus failed to amplify at least one of the genes. The results indicated that both aflatoxigenic and non-aflatoxigenic A. flavus could survive in imported peanuts and, thus, appropriate storage conditions preferably with low temperature should be considered to avoid the re-emergence of aflatoxigenic A. flavus and the subsequent aflatoxin production in peanuts during storage. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38280/1/38280.pdf Mahror, Norlia and Selamat, Jinap and Mahmud @ Ab Rashid, Nor Khaizura and Radu, Son and Chin, Cheow Keat and Samsudin, Nik Iskandar Putra and Abdul Halim, Farawahida (2019) Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia. Toxins, 11 (9). art. no. 501. pp. 1-20. ISSN 2072-6651 https://www.mdpi.com/2072-6651/11/9/501 10.3390/toxins11090501
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Peanuts are widely consumed in many local dishes in southeast Asian countries, especially in Malaysia which is one of the major peanut-importing countries in this region. Therefore, Aspergillus spp. and aflatoxin contamination in peanuts during storage are becoming major concerns due to the tropical weather in this region that favours the growth of aflatoxigenic fungi. The present study thus aimed to molecularly identify and characterise the Aspergillus section Flavi isolated from imported peanuts in Malaysia. The internal transcribed spacer (ITS) and β-tubulin sequences were used to confirm the species and determine the phylogenetic relationship among the isolates, while aflatoxin biosynthesis genes (aflR, aflP (omtA), aflD (nor-1), aflM (ver-1), and pksA) were targeted in a multiplex PCR to determine the toxigenic potential. A total of 76 and one isolates were confirmed as A. flavus and A. tamarii, respectively. The Maximum Likelihood (ML) phylogenetic tree resolved the species into two different clades in which all A. flavus (both aflatoxigenic and non-aflatoxigenic) were grouped in the same clade and A. tamarii was grouped in a different clade. The aflatoxin biosynthesis genes were detected in all aflatoxigenic A. flavus while the non-aflatoxigenic A. flavus failed to amplify at least one of the genes. The results indicated that both aflatoxigenic and non-aflatoxigenic A. flavus could survive in imported peanuts and, thus, appropriate storage conditions preferably with low temperature should be considered to avoid the re-emergence of aflatoxigenic A. flavus and the subsequent aflatoxin production in peanuts during storage.
format	Article
author	Mahror, Norlia Selamat, Jinap Mahmud @ Ab Rashid, Nor Khaizura Radu, Son Chin, Cheow Keat Samsudin, Nik Iskandar Putra Abdul Halim, Farawahida
spellingShingle	Mahror, Norlia Selamat, Jinap Mahmud @ Ab Rashid, Nor Khaizura Radu, Son Chin, Cheow Keat Samsudin, Nik Iskandar Putra Abdul Halim, Farawahida Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia
author_facet	Mahror, Norlia Selamat, Jinap Mahmud @ Ab Rashid, Nor Khaizura Radu, Son Chin, Cheow Keat Samsudin, Nik Iskandar Putra Abdul Halim, Farawahida
author_sort	Mahror, Norlia
title	Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia
title_short	Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia
title_full	Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia
title_fullStr	Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia
title_full_unstemmed	Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia
title_sort	molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of aspergillus section flavi isolated from imported peanuts along the supply chain in malaysia
publisher	MDPI
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/38280/1/38280.pdf http://psasir.upm.edu.my/id/eprint/38280/ https://www.mdpi.com/2072-6651/11/9/501
_version_	1665895971057303552
score	13.211869

Molecular characterisation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section Flavi isolated from imported peanuts along the supply chain in Malaysia

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