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Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition

The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze...

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Main Authors: Cheah, Yoke Kqueen, Cheng, Ri Wen, Yeap, Swee Keong, Khoo, Chai Hoon, See, H. S.
Format: Article
Language:English
Published: Fundacao de Pesquisas Cientificas de Ribeirao Preto 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36453/1/Analysis%20of%20TP53%20gene%20expression%20and%20p53%20level%20of%20human%20hypopharyngeal%20FaDu.pdf
http://psasir.upm.edu.my/id/eprint/36453/
http://www.geneticsmr.com/articles/2796
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spelling my.upm.eprints.364532015-09-07T07:24:17Z http://psasir.upm.edu.my/id/eprint/36453/ Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition Cheah, Yoke Kqueen Cheng, Ri Wen Yeap, Swee Keong Khoo, Chai Hoon See, H. S. The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze TP53 gene expression using real-time PCR and to determine changes in intracellular p53 level by flow cytometry after downregulation of miRNA-181a miRNA inhibitor in the FaDu cell line. TP53 gene expression showed a 3-fold increment and the p53 protein level was also increased in the miRNA-181a-treated cells. In conclusion, miRNA-181a binds to the TP53 gene and inhibits its expression, decreasing the synthesis of p53. Fundacao de Pesquisas Cientificas de Ribeirao Preto 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36453/1/Analysis%20of%20TP53%20gene%20expression%20and%20p53%20level%20of%20human%20hypopharyngeal%20FaDu.pdf Cheah, Yoke Kqueen and Cheng, Ri Wen and Yeap, Swee Keong and Khoo, Chai Hoon and See, H. S. (2014) Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition. Genetics and Molecular Research, 13 (1). pp. 1679-1683. ISSN 1676-5680 http://www.geneticsmr.com/articles/2796 10.4238/2014.January.22.4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze TP53 gene expression using real-time PCR and to determine changes in intracellular p53 level by flow cytometry after downregulation of miRNA-181a miRNA inhibitor in the FaDu cell line. TP53 gene expression showed a 3-fold increment and the p53 protein level was also increased in the miRNA-181a-treated cells. In conclusion, miRNA-181a binds to the TP53 gene and inhibits its expression, decreasing the synthesis of p53.
format Article
author Cheah, Yoke Kqueen
Cheng, Ri Wen
Yeap, Swee Keong
Khoo, Chai Hoon
See, H. S.
spellingShingle Cheah, Yoke Kqueen
Cheng, Ri Wen
Yeap, Swee Keong
Khoo, Chai Hoon
See, H. S.
Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
author_facet Cheah, Yoke Kqueen
Cheng, Ri Wen
Yeap, Swee Keong
Khoo, Chai Hoon
See, H. S.
author_sort Cheah, Yoke Kqueen
title Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
title_short Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
title_full Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
title_fullStr Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
title_full_unstemmed Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
title_sort analysis of tp53 gene expression and p53 level of human hypopharyngeal fadu (htb-43) head and neck cancer cell line after microrna-181a inhibition
publisher Fundacao de Pesquisas Cientificas de Ribeirao Preto
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36453/1/Analysis%20of%20TP53%20gene%20expression%20and%20p53%20level%20of%20human%20hypopharyngeal%20FaDu.pdf
http://psasir.upm.edu.my/id/eprint/36453/
http://www.geneticsmr.com/articles/2796
_version_ 1643831743425282048
score 13.211869

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