Analysis of TP53 gene expression and p53 level of human hypopharyngeal FaDu (HTB-43) head and neck cancer cell line after microRNA-181a inhibition
The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundacao de Pesquisas Cientificas de Ribeirao Preto
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36453/1/Analysis%20of%20TP53%20gene%20expression%20and%20p53%20level%20of%20human%20hypopharyngeal%20FaDu.pdf http://psasir.upm.edu.my/id/eprint/36453/ http://www.geneticsmr.com/articles/2796 |
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| Summary: | The identification of new biomarkers for early detection of highly recurrent head and neck cancer is urgently needed. MicroRNAs (miRNAs) are small and non-coding RNAs that regulate cancer-related gene expression, such as tumor protein 53 (TP53) gene expression. This study was carried out to analyze TP53 gene expression using real-time PCR and to determine changes in intracellular p53 level by flow cytometry after downregulation of miRNA-181a miRNA inhibitor in the FaDu cell line. TP53 gene expression showed a 3-fold increment and the p53 protein level was also increased in the miRNA-181a-treated cells. In conclusion, miRNA-181a binds to the TP53 gene and inhibits its expression, decreasing the synthesis of p53. |
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