Cover Image

Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei

In this study, vegetative cell suspensions of two Bacillus subtilis strains, L10 and G1 in equal proportions, was administered at two different doses 105 (BM5) and 108 (BM8) CFU ml−1 in the rearing water of shrimp (Litopenaeus vannamei) for eight weeks. Both probiotic groups showed a significant red...

Full description

Saved in:
Bibliographic Details
Main Authors: Zokaeifar, Hadi, Babaei, Nahid, Saad, Che Roos, Kamarudin, Mohd Salleh, Sijam, Kamaruzaman, Balcazar, Jose Luis
Format: Article
Language:English
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36331/1/Administration%20of%20Bacillus%20subtilis%20strains%20in%20the%20rearing%20water%20enhances%20the%20water%20quality.pdf
http://psasir.upm.edu.my/id/eprint/36331/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.36331
record_format eprints
spelling my.upm.eprints.363312015-11-11T06:40:28Z http://psasir.upm.edu.my/id/eprint/36331/ Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei Zokaeifar, Hadi Babaei, Nahid Saad, Che Roos Kamarudin, Mohd Salleh Sijam, Kamaruzaman Balcazar, Jose Luis In this study, vegetative cell suspensions of two Bacillus subtilis strains, L10 and G1 in equal proportions, was administered at two different doses 105 (BM5) and 108 (BM8) CFU ml−1 in the rearing water of shrimp (Litopenaeus vannamei) for eight weeks. Both probiotic groups showed a significant reduction of ammonia, nitrite and nitrate ions under in vitro and in vivo conditions. In comparison to untreated control group, final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR) and digestive enzymatic activity were significantly greater in the BM5 and BM8 groups. Significant differences for survival were recorded in the BM8 group as compared to the control. Eight weeks after the start of experiment, shrimp were challenged with Vibrio harveyi. Statistical analysis revealed significant differences in shrimp survival between probiotic and control groups. Cumulative mortality of the control group was 80%, whereas cumulative mortality of the shrimp that had been given probiotics was 36.7% with MB8 and 50% with MB5. Subsequently, real-time RT-PCR was employed to determine the mRNA levels of prophenoloxidase (proPO), peroxinectin (PE), lipopolysaccharide- and β-1,3-glucan- binding protein (LGBP) and serine protein (SP). The expression of all immune-related genes studied was only significantly up-regulated in the BM5 group compared to the BM8 and control groups. These results suggest that administration of B. subtilis strains in the rearing water confers beneficial effects for shrimp aquaculture, considering water quality, growth performance, digestive enzymatic activity, immune response and disease resistance. Elsevier 2014-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36331/1/Administration%20of%20Bacillus%20subtilis%20strains%20in%20the%20rearing%20water%20enhances%20the%20water%20quality.pdf Zokaeifar, Hadi and Babaei, Nahid and Saad, Che Roos and Kamarudin, Mohd Salleh and Sijam, Kamaruzaman and Balcazar, Jose Luis (2014) Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 36 (1). pp. 68-74. ISSN 1050-4648; ESSN: 1095-9947 10.1016/j.fsi.2013.10.007
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In this study, vegetative cell suspensions of two Bacillus subtilis strains, L10 and G1 in equal proportions, was administered at two different doses 105 (BM5) and 108 (BM8) CFU ml−1 in the rearing water of shrimp (Litopenaeus vannamei) for eight weeks. Both probiotic groups showed a significant reduction of ammonia, nitrite and nitrate ions under in vitro and in vivo conditions. In comparison to untreated control group, final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR) and digestive enzymatic activity were significantly greater in the BM5 and BM8 groups. Significant differences for survival were recorded in the BM8 group as compared to the control. Eight weeks after the start of experiment, shrimp were challenged with Vibrio harveyi. Statistical analysis revealed significant differences in shrimp survival between probiotic and control groups. Cumulative mortality of the control group was 80%, whereas cumulative mortality of the shrimp that had been given probiotics was 36.7% with MB8 and 50% with MB5. Subsequently, real-time RT-PCR was employed to determine the mRNA levels of prophenoloxidase (proPO), peroxinectin (PE), lipopolysaccharide- and β-1,3-glucan- binding protein (LGBP) and serine protein (SP). The expression of all immune-related genes studied was only significantly up-regulated in the BM5 group compared to the BM8 and control groups. These results suggest that administration of B. subtilis strains in the rearing water confers beneficial effects for shrimp aquaculture, considering water quality, growth performance, digestive enzymatic activity, immune response and disease resistance.
format Article
author Zokaeifar, Hadi
Babaei, Nahid
Saad, Che Roos
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Balcazar, Jose Luis
spellingShingle Zokaeifar, Hadi
Babaei, Nahid
Saad, Che Roos
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Balcazar, Jose Luis
Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei
author_facet Zokaeifar, Hadi
Babaei, Nahid
Saad, Che Roos
Kamarudin, Mohd Salleh
Sijam, Kamaruzaman
Balcazar, Jose Luis
author_sort Zokaeifar, Hadi
title Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei
title_short Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei
title_full Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei
title_fullStr Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei
title_full_unstemmed Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei
title_sort administration of bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against vibrio harveyi infection in juvenile white shrimp, litopenaeus vannamei
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36331/1/Administration%20of%20Bacillus%20subtilis%20strains%20in%20the%20rearing%20water%20enhances%20the%20water%20quality.pdf
http://psasir.upm.edu.my/id/eprint/36331/
_version_ 1643831710752702464
score 13.211869

Similar Items