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A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments

The present study examined the effect of different heat treatments; (1) chilled, (2) boiled at 100°C for 30 min, and (3) autoclaved at 121°C at 15 psi for 20 min, on the expression of goat skeletal muscle proteins using two-dimensional gel electrophoresis. The molecular weight (MW) and isoelectric p...

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Main Authors: Zainal Abidin, Siti Aimi Sarah, Karsani, Saiful Anuar, Ismail, Amin, Khairil Mokhtar, Nur Fadhilah, Sazili, Awis Qurni
Format: Article
Language:English
Published: Pakistan Agricultural Scientists Forum 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36235/1/A%20proteomic%20based%20assessment%20on%20changes%20in%20myofirbrillar%20proteins%20of%20goat%20longissimus%20muscle%20as%20affected%20by%20heat%20treatments.pdf
http://psasir.upm.edu.my/id/eprint/36235/
http://www.thejaps.org.pk/Volume/2014/24-2/index.php
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spelling my.upm.eprints.362352015-11-11T07:04:05Z http://psasir.upm.edu.my/id/eprint/36235/ A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments Zainal Abidin, Siti Aimi Sarah Karsani, Saiful Anuar Ismail, Amin Khairil Mokhtar, Nur Fadhilah Sazili, Awis Qurni The present study examined the effect of different heat treatments; (1) chilled, (2) boiled at 100°C for 30 min, and (3) autoclaved at 121°C at 15 psi for 20 min, on the expression of goat skeletal muscle proteins using two-dimensional gel electrophoresis. The molecular weight (MW) and isoelectric point (pI) of heat stable proteins were characterised followed by identification of the proteins by MALDI-TOF/TOF mass spectrometry. There were 153 protein spots obtained in the boiled samples, while only 46 protein spots were observed in the autoclaved samples. Thirteen spots that exhibited high intensity of protein were chosen from the autoclaved sample for MALDI-TOF/TOF mass spectrometry analysis. The putative heat stable proteins identified were myosin light chain (MLC), actin, tropomyosin (TPM), troponin T (TnT), myoglobin, and creatine kinase. The Proc-GLM analysis revealed that the heat treatments have resulted in significant differences in spot intensities of actin, troponin T (TnT), myoglobin, and creatine kinase with no significant changes noted in other proteins. Pakistan Agricultural Scientists Forum 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36235/1/A%20proteomic%20based%20assessment%20on%20changes%20in%20myofirbrillar%20proteins%20of%20goat%20longissimus%20muscle%20as%20affected%20by%20heat%20treatments.pdf Zainal Abidin, Siti Aimi Sarah and Karsani, Saiful Anuar and Ismail, Amin and Khairil Mokhtar, Nur Fadhilah and Sazili, Awis Qurni (2014) A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments. The Journal of Animal and Plant Sciences, 24 (2). pp. 406-412. ISSN 1018-7081 http://www.thejaps.org.pk/Volume/2014/24-2/index.php
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present study examined the effect of different heat treatments; (1) chilled, (2) boiled at 100°C for 30 min, and (3) autoclaved at 121°C at 15 psi for 20 min, on the expression of goat skeletal muscle proteins using two-dimensional gel electrophoresis. The molecular weight (MW) and isoelectric point (pI) of heat stable proteins were characterised followed by identification of the proteins by MALDI-TOF/TOF mass spectrometry. There were 153 protein spots obtained in the boiled samples, while only 46 protein spots were observed in the autoclaved samples. Thirteen spots that exhibited high intensity of protein were chosen from the autoclaved sample for MALDI-TOF/TOF mass spectrometry analysis. The putative heat stable proteins identified were myosin light chain (MLC), actin, tropomyosin (TPM), troponin T (TnT), myoglobin, and creatine kinase. The Proc-GLM analysis revealed that the heat treatments have resulted in significant differences in spot intensities of actin, troponin T (TnT), myoglobin, and creatine kinase with no significant changes noted in other proteins.
format Article
author Zainal Abidin, Siti Aimi Sarah
Karsani, Saiful Anuar
Ismail, Amin
Khairil Mokhtar, Nur Fadhilah
Sazili, Awis Qurni
spellingShingle Zainal Abidin, Siti Aimi Sarah
Karsani, Saiful Anuar
Ismail, Amin
Khairil Mokhtar, Nur Fadhilah
Sazili, Awis Qurni
A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
author_facet Zainal Abidin, Siti Aimi Sarah
Karsani, Saiful Anuar
Ismail, Amin
Khairil Mokhtar, Nur Fadhilah
Sazili, Awis Qurni
author_sort Zainal Abidin, Siti Aimi Sarah
title A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
title_short A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
title_full A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
title_fullStr A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
title_full_unstemmed A proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
title_sort proteomic based assessment on changes in myofirbrillar proteins of goat longissimus muscle as affected by heat treatments
publisher Pakistan Agricultural Scientists Forum
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36235/1/A%20proteomic%20based%20assessment%20on%20changes%20in%20myofirbrillar%20proteins%20of%20goat%20longissimus%20muscle%20as%20affected%20by%20heat%20treatments.pdf
http://psasir.upm.edu.my/id/eprint/36235/
http://www.thejaps.org.pk/Volume/2014/24-2/index.php
_version_ 1643831688017477632
score 13.211869

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