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Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells

The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells...

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Main Authors: Ismail, Norsharina, Akhtar, Muhammad Nadeem, Ismail, Maznah, Zareen, Seema, Shah, Syed Adnan Ali, Lajis, Nordin, Tajuddin, Saiful Nizam
Format: Article
Published: Taylor & Francis 2015
Online Access:http://psasir.upm.edu.my/id/eprint/34639/
http://www.tandfonline.com/doi/full/10.1080/14786419.2014.985676
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spelling my.upm.eprints.346392015-12-16T07:46:32Z http://psasir.upm.edu.my/id/eprint/34639/ Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells Ismail, Norsharina Akhtar, Muhammad Nadeem Ismail, Maznah Zareen, Seema Shah, Syed Adnan Ali Lajis, Nordin Tajuddin, Saiful Nizam The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1–42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Aβ1–42-induced cell death. Flavonoids (3–6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. Taylor & Francis 2015 Article PeerReviewed Ismail, Norsharina and Akhtar, Muhammad Nadeem and Ismail, Maznah and Zareen, Seema and Shah, Syed Adnan Ali and Lajis, Nordin and Tajuddin, Saiful Nizam (2015) Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells. Natural Product Research, 29 (16). pp. 1571-1574. ISSN 1478-6419; ESSN: 1478-6427 http://www.tandfonline.com/doi/full/10.1080/14786419.2014.985676 10.1080/14786419.2014.985676
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1–42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Aβ1–42-induced cell death. Flavonoids (3–6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects.
format Article
author Ismail, Norsharina
Akhtar, Muhammad Nadeem
Ismail, Maznah
Zareen, Seema
Shah, Syed Adnan Ali
Lajis, Nordin
Tajuddin, Saiful Nizam
spellingShingle Ismail, Norsharina
Akhtar, Muhammad Nadeem
Ismail, Maznah
Zareen, Seema
Shah, Syed Adnan Ali
Lajis, Nordin
Tajuddin, Saiful Nizam
Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
author_facet Ismail, Norsharina
Akhtar, Muhammad Nadeem
Ismail, Maznah
Zareen, Seema
Shah, Syed Adnan Ali
Lajis, Nordin
Tajuddin, Saiful Nizam
author_sort Ismail, Norsharina
title Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
title_short Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
title_full Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
title_fullStr Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
title_full_unstemmed Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells
title_sort neuroprotective effect from stem bark extracts of knema laurina against h₂o₂- and aβ(1-42)-induced cell death in human sh-sy5y cells
publisher Taylor & Francis
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/34639/
http://www.tandfonline.com/doi/full/10.1080/14786419.2014.985676
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score 13.211869

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