Cover Image

Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391

One of the basic causes of mortality in developed countries is cardiovascular disease,especially coronary heart disease. Control of blood cholesterol level is important to avoid cardiovascular disease. In this study the bioactive compound(s) of Monascus purpureus FTC5391 with hypocholesterolemic po...

Full description

Saved in:
Bibliographic Details
Main Author: Ajdari, Zahra
Format: Thesis
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/33315/1/FBSB%202012%2022R.pdf
http://psasir.upm.edu.my/id/eprint/33315/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.33315
record_format eprints
spelling my.upm.eprints.333152015-03-11T03:59:49Z http://psasir.upm.edu.my/id/eprint/33315/ Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391 Ajdari, Zahra One of the basic causes of mortality in developed countries is cardiovascular disease,especially coronary heart disease. Control of blood cholesterol level is important to avoid cardiovascular disease. In this study the bioactive compound(s) of Monascus purpureus FTC5391 with hypocholesterolemic potential was investigated. In order to purify and identify the hypocholesterolemic bioactive agent of M. purpureus FTC5391 fermented product, cultivated via submerged fermentation in modified medium was sub-fractionated by solid phase extraction (SPE). The hypocholesterolemic active fraction in rats was selected for further purification by recycling preparative HPLC (RP-HPLC). The effective fraction significantly (P-value < 0.05) regulated the serum lipid-profile of hypercholesterolemic rats as compared with no treated control groups. This fraction increased the serum HDL-C (100%) and decreased the TC (42.6%), LDL-C (46%), TG (54.4%) levels as well as TC/HDL-C ratio (71.9%). Whereas, Atrovastatin, as a positive control, could regulate the rat serum lipid profile by increasing the serum HDL-C (37.5%) and decreasing the serum TC, LDL-C, TG levels and TC/HDL-C ratio 45.15%, 42%, 54.3% and 61.4%, respectively. MS spectrum of the pure effective fraction and its comparison with spectra library suggested the cyclopropane-carboxylic acid 4-dodecanoeil ester as a novel hypocholesterolemic agent. The obvious superiority of this novel compound to gulate the lipid profile in comparison to the atrovastatin and its ability to decrease the liver damage indicator enzymes, revealed the golden perspective of this novel molecule as a potent future hypocholesterolemic drug. 2012-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33315/1/FBSB%202012%2022R.pdf Ajdari, Zahra (2012) Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391. PhD thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description One of the basic causes of mortality in developed countries is cardiovascular disease,especially coronary heart disease. Control of blood cholesterol level is important to avoid cardiovascular disease. In this study the bioactive compound(s) of Monascus purpureus FTC5391 with hypocholesterolemic potential was investigated. In order to purify and identify the hypocholesterolemic bioactive agent of M. purpureus FTC5391 fermented product, cultivated via submerged fermentation in modified medium was sub-fractionated by solid phase extraction (SPE). The hypocholesterolemic active fraction in rats was selected for further purification by recycling preparative HPLC (RP-HPLC). The effective fraction significantly (P-value < 0.05) regulated the serum lipid-profile of hypercholesterolemic rats as compared with no treated control groups. This fraction increased the serum HDL-C (100%) and decreased the TC (42.6%), LDL-C (46%), TG (54.4%) levels as well as TC/HDL-C ratio (71.9%). Whereas, Atrovastatin, as a positive control, could regulate the rat serum lipid profile by increasing the serum HDL-C (37.5%) and decreasing the serum TC, LDL-C, TG levels and TC/HDL-C ratio 45.15%, 42%, 54.3% and 61.4%, respectively. MS spectrum of the pure effective fraction and its comparison with spectra library suggested the cyclopropane-carboxylic acid 4-dodecanoeil ester as a novel hypocholesterolemic agent. The obvious superiority of this novel compound to gulate the lipid profile in comparison to the atrovastatin and its ability to decrease the liver damage indicator enzymes, revealed the golden perspective of this novel molecule as a potent future hypocholesterolemic drug.
format Thesis
author Ajdari, Zahra
spellingShingle Ajdari, Zahra
Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
author_facet Ajdari, Zahra
author_sort Ajdari, Zahra
title Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_short Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_full Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_fullStr Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_full_unstemmed Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_sort isolation and characterization of hypocholesterolemic bioactive agent extracted from monascus purpureus ftc5391
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/33315/1/FBSB%202012%2022R.pdf
http://psasir.upm.edu.my/id/eprint/33315/
_version_ 1643830838824009728
score 13.211869

Similar Items