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A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway

The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)- 3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear...

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Main Authors: Anasamy, Theebaa, Abdul, Ahmad Bustamam, Sukari, Mohd Aspollah, Abdelwahab, Siddig Ibrahim, Mohan, Syam, Kamalidehghan, Behnam, Azid, Mohd Zulkhairi, Muhammad Nadzri, Nabilah, Andas, A. Reenaa Joys, Beng, Ng Kuan, A. Hadi, A. Hamid, Rahman, Heshu Sulaiman
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30040/1/A%20phenylbutenoid%20dimer.pdf
http://psasir.upm.edu.my/id/eprint/30040/
http://dx.doi.org/10.1155/2013/939810
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spelling my.upm.eprints.300402016-02-12T08:13:18Z http://psasir.upm.edu.my/id/eprint/30040/ A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway Anasamy, Theebaa Abdul, Ahmad Bustamam Sukari, Mohd Aspollah Abdelwahab, Siddig Ibrahim Mohan, Syam Kamalidehghan, Behnam Azid, Mohd Zulkhairi Muhammad Nadzri, Nabilah Andas, A. Reenaa Joys Beng, Ng Kuan A. Hadi, A. Hamid Rahman, Heshu Sulaiman The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)- 3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear cells, and to further investigate the involvement of apoptosis-related proteins that leads, to the probable pathway in which apoptosis is triggered. Cytotoxicity test using MTT assay showed selective inhibition of ZC-B11 towards T-acute lymphoblastic leukemia cells, CEMss, with an ICvalue of 7.11 ± 0.240 g/mL, which did not reveal cytotoxic effects towards normal human blood mononuclear cells (IC> 50 g/mL). Morphology assessments demonstrated distinctive morphological changes corresponding to a typical apoptosis. ZC-B11 also arrested cell cycle progression at S phase and causes DNA fragmentation in CEMss cells. Decline of mitochondrial membrane potential was also determined qualitatively. In the apoptosis-related protein determination, ZC-B11 was found to significantly upregulate Bax, caspase 3/7, caspase 9, cytochrome c, and SMAC and downregulate Bcl-2, HSP70, and XIAP, but did not affect caspase 8, p53, and BID. These results demonstrated for the first time the apoptogenic property of ZC-B11 on CEMss cell line, leading to the programmed cell death via intrinsic mitochondrial pathway of apoptosis induction. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30040/1/A%20phenylbutenoid%20dimer.pdf Anasamy, Theebaa and Abdul, Ahmad Bustamam and Sukari, Mohd Aspollah and Abdelwahab, Siddig Ibrahim and Mohan, Syam and Kamalidehghan, Behnam and Azid, Mohd Zulkhairi and Muhammad Nadzri, Nabilah and Andas, A. Reenaa Joys and Beng, Ng Kuan and A. Hadi, A. Hamid and Rahman, Heshu Sulaiman (2013) A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway. Evidence-Based Complementary and Alternative Medicine, 2013 (939810). pp. 1-14. ISSN 1741-427X; ESSN: 1741-4288 http://dx.doi.org/10.1155/2013/939810 10.1155/2013/939810
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)- 3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear cells, and to further investigate the involvement of apoptosis-related proteins that leads, to the probable pathway in which apoptosis is triggered. Cytotoxicity test using MTT assay showed selective inhibition of ZC-B11 towards T-acute lymphoblastic leukemia cells, CEMss, with an ICvalue of 7.11 ± 0.240 g/mL, which did not reveal cytotoxic effects towards normal human blood mononuclear cells (IC> 50 g/mL). Morphology assessments demonstrated distinctive morphological changes corresponding to a typical apoptosis. ZC-B11 also arrested cell cycle progression at S phase and causes DNA fragmentation in CEMss cells. Decline of mitochondrial membrane potential was also determined qualitatively. In the apoptosis-related protein determination, ZC-B11 was found to significantly upregulate Bax, caspase 3/7, caspase 9, cytochrome c, and SMAC and downregulate Bcl-2, HSP70, and XIAP, but did not affect caspase 8, p53, and BID. These results demonstrated for the first time the apoptogenic property of ZC-B11 on CEMss cell line, leading to the programmed cell death via intrinsic mitochondrial pathway of apoptosis induction.
format Article
author Anasamy, Theebaa
Abdul, Ahmad Bustamam
Sukari, Mohd Aspollah
Abdelwahab, Siddig Ibrahim
Mohan, Syam
Kamalidehghan, Behnam
Azid, Mohd Zulkhairi
Muhammad Nadzri, Nabilah
Andas, A. Reenaa Joys
Beng, Ng Kuan
A. Hadi, A. Hamid
Rahman, Heshu Sulaiman
spellingShingle Anasamy, Theebaa
Abdul, Ahmad Bustamam
Sukari, Mohd Aspollah
Abdelwahab, Siddig Ibrahim
Mohan, Syam
Kamalidehghan, Behnam
Azid, Mohd Zulkhairi
Muhammad Nadzri, Nabilah
Andas, A. Reenaa Joys
Beng, Ng Kuan
A. Hadi, A. Hamid
Rahman, Heshu Sulaiman
A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
author_facet Anasamy, Theebaa
Abdul, Ahmad Bustamam
Sukari, Mohd Aspollah
Abdelwahab, Siddig Ibrahim
Mohan, Syam
Kamalidehghan, Behnam
Azid, Mohd Zulkhairi
Muhammad Nadzri, Nabilah
Andas, A. Reenaa Joys
Beng, Ng Kuan
A. Hadi, A. Hamid
Rahman, Heshu Sulaiman
author_sort Anasamy, Theebaa
title A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
title_short A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
title_full A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
title_fullStr A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
title_full_unstemmed A phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in T-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
title_sort phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(e)-3′′′,4′′′-dimethoxystyryl] cyclohex-1-ene, exhibits apoptogenic properties in t-acute lymphoblastic leukemia cells via induction of p53-independent mitochondrial signalling pathway
publisher Hindawi Publishing Corporation
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30040/1/A%20phenylbutenoid%20dimer.pdf
http://psasir.upm.edu.my/id/eprint/30040/
http://dx.doi.org/10.1155/2013/939810
_version_ 1643829939143704576
score 13.211869

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