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Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways

The anti-cancer effect of Boesenbergin A (BA) isolated from Boesenbergia rotunda, via the induction of apoptosis resulting from mitochondrial dysfunction was assessed in human non-small cell lung cancer (A549) cells. The apoptotic mechanisms of BA induction on cancer cells were studied in the presen...

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Main Authors: Mohd Isa, Norbaiti, Abdul, Ahmad Bustamam, Abdelwahab, Siddig Ibrahim, Abdullah, Rasedee, Sukari, Mohd Aspollah, Kamalidehghan, Behnam, A. Hadi, A. Hamid, Mohan, Syam
Format: Article
Language:English
English
Published: Elsevier 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30035/1/Boesenbergin%20A.pdf
http://psasir.upm.edu.my/id/eprint/30035/
http://www.sciencedirect.com/science/journal/17564646/5/1
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spelling my.upm.eprints.300352015-10-08T00:12:36Z http://psasir.upm.edu.my/id/eprint/30035/ Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways Mohd Isa, Norbaiti Abdul, Ahmad Bustamam Abdelwahab, Siddig Ibrahim Abdullah, Rasedee Sukari, Mohd Aspollah Kamalidehghan, Behnam A. Hadi, A. Hamid Mohan, Syam The anti-cancer effect of Boesenbergin A (BA) isolated from Boesenbergia rotunda, via the induction of apoptosis resulting from mitochondrial dysfunction was assessed in human non-small cell lung cancer (A549) cells. The apoptotic mechanisms of BA induction on cancer cells were studied in the present study for the first time. Nuclear stain, measuring the accumulation of sub-G1 cell population and DNA ladder were done to determine the apoptosis. Further investigations into the depletion of mitochondrial membrane potential and release of cytochrome c determined that BA treatment induced apoptosis via the regulation of the expression of pro-survival and pro-apoptotic Bcl-2 family members. The involvement of both intrinsic and extrinsic caspases (caspase 3/7, 9 and 8) were significantly increased. Moreover the role of free radicals was significantly found to be elevated with concomitant decrease in HSP70. In conclusion the results from the current study indicated BA could be a promising agent for the treatment of lung cancer. Elsevier 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30035/1/Boesenbergin%20A.pdf Mohd Isa, Norbaiti and Abdul, Ahmad Bustamam and Abdelwahab, Siddig Ibrahim and Abdullah, Rasedee and Sukari, Mohd Aspollah and Kamalidehghan, Behnam and A. Hadi, A. Hamid and Mohan, Syam (2013) Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways. Journal of Functional Foods, 5 (1). pp. 87-97. ISSN 1756-4646 http://www.sciencedirect.com/science/journal/17564646/5/1 10.1016/j.jff.2012.08.008 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The anti-cancer effect of Boesenbergin A (BA) isolated from Boesenbergia rotunda, via the induction of apoptosis resulting from mitochondrial dysfunction was assessed in human non-small cell lung cancer (A549) cells. The apoptotic mechanisms of BA induction on cancer cells were studied in the present study for the first time. Nuclear stain, measuring the accumulation of sub-G1 cell population and DNA ladder were done to determine the apoptosis. Further investigations into the depletion of mitochondrial membrane potential and release of cytochrome c determined that BA treatment induced apoptosis via the regulation of the expression of pro-survival and pro-apoptotic Bcl-2 family members. The involvement of both intrinsic and extrinsic caspases (caspase 3/7, 9 and 8) were significantly increased. Moreover the role of free radicals was significantly found to be elevated with concomitant decrease in HSP70. In conclusion the results from the current study indicated BA could be a promising agent for the treatment of lung cancer.
format Article
author Mohd Isa, Norbaiti
Abdul, Ahmad Bustamam
Abdelwahab, Siddig Ibrahim
Abdullah, Rasedee
Sukari, Mohd Aspollah
Kamalidehghan, Behnam
A. Hadi, A. Hamid
Mohan, Syam
spellingShingle Mohd Isa, Norbaiti
Abdul, Ahmad Bustamam
Abdelwahab, Siddig Ibrahim
Abdullah, Rasedee
Sukari, Mohd Aspollah
Kamalidehghan, Behnam
A. Hadi, A. Hamid
Mohan, Syam
Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways
author_facet Mohd Isa, Norbaiti
Abdul, Ahmad Bustamam
Abdelwahab, Siddig Ibrahim
Abdullah, Rasedee
Sukari, Mohd Aspollah
Kamalidehghan, Behnam
A. Hadi, A. Hamid
Mohan, Syam
author_sort Mohd Isa, Norbaiti
title Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways
title_short Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways
title_full Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways
title_fullStr Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways
title_full_unstemmed Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro : involvement of HSP70 and Bcl2/Bax signalling pathways
title_sort boesenbergin a, a chalcone from boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in a549 cells in vitro : involvement of hsp70 and bcl2/bax signalling pathways
publisher Elsevier
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30035/1/Boesenbergin%20A.pdf
http://psasir.upm.edu.my/id/eprint/30035/
http://www.sciencedirect.com/science/journal/17564646/5/1
_version_ 1643829937606492160
score 13.211869

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