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Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia

Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public confidence problem over shellfish safety and may result in important economic losses for th...

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Main Authors: Md. Nordin, Wan Norhana, A. Rahim, Masazurah, Mahyudin, Nor Ainy, I., Ismail
Format: Article
Language:English
English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf
http://psasir.upm.edu.my/id/eprint/24050/
http://www.ifrj.upm.edu.my/volume-18-2011.html
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spelling my.upm.eprints.240502015-06-02T08:23:05Z http://psasir.upm.edu.my/id/eprint/24050/ Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia Md. Nordin, Wan Norhana A. Rahim, Masazurah Mahyudin, Nor Ainy I., Ismail Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public confidence problem over shellfish safety and may result in important economic losses for the seafood industry. The work presented in this study investigated the presence of HAV in shellfish from Peninsular Malaysia. A total of 365 of cultured and wild shellfish from 36 sampling locations located throughout Peninsular Malaysia were examined using a commercial nucleic acid extraction and reverse transcription -polymerase chain reaction (RT-PCR) kit. HAV was not detected in almost all of the shellfish samples examined. Only one cockle sample from Changkat, Seberang Perai was positive for HAV. The results suggest the absence of HAV or very low amount of HAV viral particles in most of the shellfish examined. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf Md. Nordin, Wan Norhana and A. Rahim, Masazurah and Mahyudin, Nor Ainy and I., Ismail (2011) Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia. International Food Research Journal, 18 (1). pp. 411-415. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public confidence problem over shellfish safety and may result in important economic losses for the seafood industry. The work presented in this study investigated the presence of HAV in shellfish from Peninsular Malaysia. A total of 365 of cultured and wild shellfish from 36 sampling locations located throughout Peninsular Malaysia were examined using a commercial nucleic acid extraction and reverse transcription -polymerase chain reaction (RT-PCR) kit. HAV was not detected in almost all of the shellfish samples examined. Only one cockle sample from Changkat, Seberang Perai was positive for HAV. The results suggest the absence of HAV or very low amount of HAV viral particles in most of the shellfish examined.
format Article
author Md. Nordin, Wan Norhana
A. Rahim, Masazurah
Mahyudin, Nor Ainy
I., Ismail
spellingShingle Md. Nordin, Wan Norhana
A. Rahim, Masazurah
Mahyudin, Nor Ainy
I., Ismail
Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
author_facet Md. Nordin, Wan Norhana
A. Rahim, Masazurah
Mahyudin, Nor Ainy
I., Ismail
author_sort Md. Nordin, Wan Norhana
title Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
title_short Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
title_full Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
title_fullStr Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
title_full_unstemmed Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
title_sort reverse transcriptase pcr detection of hepatitis a virus (hav) in cultured and wild shellfish from the peninsular of malaysia
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf
http://psasir.upm.edu.my/id/eprint/24050/
http://www.ifrj.upm.edu.my/volume-18-2011.html
_version_ 1643828244659568640
score 13.211869

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