Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public confidence problem over shellfish safety and may result in important economic losses for th...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf http://psasir.upm.edu.my/id/eprint/24050/ http://www.ifrj.upm.edu.my/volume-18-2011.html |
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| Summary: | Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A
have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public
confidence problem over shellfish safety and may result in important economic losses for the seafood industry.
The work presented in this study investigated the presence of HAV in shellfish from Peninsular Malaysia. A
total of 365 of cultured and wild shellfish from 36 sampling locations located throughout Peninsular Malaysia
were examined using a commercial nucleic acid extraction and reverse transcription -polymerase chain reaction
(RT-PCR) kit. HAV was not detected in almost all of the shellfish samples examined. Only one cockle sample
from Changkat, Seberang Perai was positive for HAV. The results suggest the absence of HAV or very low
amount of HAV viral particles in most of the shellfish examined. |
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