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Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.

Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the...

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Main Authors: Muhammad Noor, Ahmad Shukri, Inami, W., Miyakawa, Atsuo, Kawata, Yoshimasa
Format: Article
Language:English
English
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23367/2/Using%20two.pdf
http://psasir.upm.edu.my/id/eprint/23367/
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spelling my.upm.eprints.233672015-10-20T05:59:36Z http://psasir.upm.edu.my/id/eprint/23367/ Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. Muhammad Noor, Ahmad Shukri Inami, W. Miyakawa, Atsuo Kawata, Yoshimasa Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the dissociation constants were used in the calculation. Agreement values of the Ca2+ from the calculation with the known Ca2+ buffer solution used for the measurement was obtained for various Ca2+ and protein concentration. The analytical analysis shows that dissociation constants for each binding’sdeviation errors are small to affect free Ca2+ determination. This would provide an accurate measurement of free Ca2+ in biological cells than the typical two emission wavelength processes. Furthermore, using two-photon excitation methods, deep inside cells observation of free Ca2+ are possible using the methods introduced. 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23367/2/Using%20two.pdf Muhammad Noor, Ahmad Shukri and Inami, W. and Miyakawa, Atsuo and Kawata, Yoshimasa (2011) Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. International Journal of the Physical Sciences, 6 (33). art. no. D06240B19089 . 7435 -7440. ISSN 1992 -1950 10.5897/IJPS11.482 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the dissociation constants were used in the calculation. Agreement values of the Ca2+ from the calculation with the known Ca2+ buffer solution used for the measurement was obtained for various Ca2+ and protein concentration. The analytical analysis shows that dissociation constants for each binding’sdeviation errors are small to affect free Ca2+ determination. This would provide an accurate measurement of free Ca2+ in biological cells than the typical two emission wavelength processes. Furthermore, using two-photon excitation methods, deep inside cells observation of free Ca2+ are possible using the methods introduced.
format Article
author Muhammad Noor, Ahmad Shukri
Inami, W.
Miyakawa, Atsuo
Kawata, Yoshimasa
spellingShingle Muhammad Noor, Ahmad Shukri
Inami, W.
Miyakawa, Atsuo
Kawata, Yoshimasa
Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
author_facet Muhammad Noor, Ahmad Shukri
Inami, W.
Miyakawa, Atsuo
Kawata, Yoshimasa
author_sort Muhammad Noor, Ahmad Shukri
title Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
title_short Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
title_full Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
title_fullStr Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
title_full_unstemmed Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
title_sort using two-photon excitation methods for determination of ca2+ in contamination with protein - full length research paper.
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/23367/2/Using%20two.pdf
http://psasir.upm.edu.my/id/eprint/23367/
_version_ 1643828037107580928
score 13.211869

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