Cover Image

Basic characteristics of cellulase immobilized on lignophenol

Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-bas...

Full description

Saved in:
Bibliographic Details
Main Authors: Nonaka, Hiroshi, Ariffin, Hidayah, Funaoka, Masamitsu
Format: Article
Language:English
Published: Nihon Kobunshi Gakkai,Society of Polymer Science, Japan 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf
http://psasir.upm.edu.my/id/eprint/22349/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.22349
record_format eprints
spelling my.upm.eprints.223492015-12-10T03:36:37Z http://psasir.upm.edu.my/id/eprint/22349/ Basic characteristics of cellulase immobilized on lignophenol Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable. Nihon Kobunshi Gakkai,Society of Polymer Science, Japan 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf Nonaka, Hiroshi and Ariffin, Hidayah and Funaoka, Masamitsu (2011) Basic characteristics of cellulase immobilized on lignophenol. Kobunshi Ronbunshu, 68 (5). pp. 315-319. ISSN 0386-2186 10.1295/koron.68.315
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable.
format Article
author Nonaka, Hiroshi
Ariffin, Hidayah
Funaoka, Masamitsu
spellingShingle Nonaka, Hiroshi
Ariffin, Hidayah
Funaoka, Masamitsu
Basic characteristics of cellulase immobilized on lignophenol
author_facet Nonaka, Hiroshi
Ariffin, Hidayah
Funaoka, Masamitsu
author_sort Nonaka, Hiroshi
title Basic characteristics of cellulase immobilized on lignophenol
title_short Basic characteristics of cellulase immobilized on lignophenol
title_full Basic characteristics of cellulase immobilized on lignophenol
title_fullStr Basic characteristics of cellulase immobilized on lignophenol
title_full_unstemmed Basic characteristics of cellulase immobilized on lignophenol
title_sort basic characteristics of cellulase immobilized on lignophenol
publisher Nihon Kobunshi Gakkai,Society of Polymer Science, Japan
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf
http://psasir.upm.edu.my/id/eprint/22349/
_version_ 1643827802354483200
score 13.211869

Similar Items