Basic characteristics of cellulase immobilized on lignophenol
Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-bas...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nihon Kobunshi Gakkai,Society of Polymer Science, Japan
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf http://psasir.upm.edu.my/id/eprint/22349/ |
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| Summary: | Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable. |
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