Cover Image

Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.

The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL−1) was much higher t...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohamad, Siti Norita, Ramanan, Ramakrishnan Nagasundara, Mohamad, Rosfarizan, Ariff, Arbakariya
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22304/1/Improved%20mannan.pdf
http://psasir.upm.edu.my/id/eprint/22304/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.22304
record_format eprints
spelling my.upm.eprints.223042015-10-20T00:11:52Z http://psasir.upm.edu.my/id/eprint/22304/ Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization. Mohamad, Siti Norita Ramanan, Ramakrishnan Nagasundara Mohamad, Rosfarizan Ariff, Arbakariya The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL−1) was much higher than those observed on other carbon substrates, locust bean gum (1148 nkat mL−1), α-cellulose (10.7 nkat mL−1), glucose (8.8 nkat mL−1) and carboxymethylcellulose (4.6 nkat mL−1). For fermentation using GG as a carbon source, bacteriological peptone gave the highest β-mannanase activity (1744 nkat mL−1) followed by peptone from meat (1168 nkat mL−1), yeast extract (817 nkat mL−1), ammonium sulphate (241 nkat mL−1), ammonium nitrate (113 nkat mL−1) and ammonium chloride (99 nkat mL−1) when used as a nitrogen source. The composition of bacteriological peptone and initial pH of the medium were further optimized using response surface methodology (RSM). Medium consisted of 21.3 g L−1 GG and 57 g L−1 peptone with initial culture pH of 5.5 was optimum for β-mannanase production (2063 nkat mL−1) by A. niger. The β-mannanase production obtained in this study using A. niger was significantly higher than those reported in the literature. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22304/1/Improved%20mannan.pdf Mohamad, Siti Norita and Ramanan, Ramakrishnan Nagasundara and Mohamad, Rosfarizan and Ariff, Arbakariya (2011) Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization. New Biotechnology, 28 (2). pp. 146-152. ISSN 1871-6784 10.1016/j.nbt.2010.10.008 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL−1) was much higher than those observed on other carbon substrates, locust bean gum (1148 nkat mL−1), α-cellulose (10.7 nkat mL−1), glucose (8.8 nkat mL−1) and carboxymethylcellulose (4.6 nkat mL−1). For fermentation using GG as a carbon source, bacteriological peptone gave the highest β-mannanase activity (1744 nkat mL−1) followed by peptone from meat (1168 nkat mL−1), yeast extract (817 nkat mL−1), ammonium sulphate (241 nkat mL−1), ammonium nitrate (113 nkat mL−1) and ammonium chloride (99 nkat mL−1) when used as a nitrogen source. The composition of bacteriological peptone and initial pH of the medium were further optimized using response surface methodology (RSM). Medium consisted of 21.3 g L−1 GG and 57 g L−1 peptone with initial culture pH of 5.5 was optimum for β-mannanase production (2063 nkat mL−1) by A. niger. The β-mannanase production obtained in this study using A. niger was significantly higher than those reported in the literature.
format Article
author Mohamad, Siti Norita
Ramanan, Ramakrishnan Nagasundara
Mohamad, Rosfarizan
Ariff, Arbakariya
spellingShingle Mohamad, Siti Norita
Ramanan, Ramakrishnan Nagasundara
Mohamad, Rosfarizan
Ariff, Arbakariya
Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
author_facet Mohamad, Siti Norita
Ramanan, Ramakrishnan Nagasundara
Mohamad, Rosfarizan
Ariff, Arbakariya
author_sort Mohamad, Siti Norita
title Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
title_short Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
title_full Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
title_fullStr Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
title_full_unstemmed Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
title_sort improved mannan-degrading enzymes' production by aspergillus niger through medium optimization.
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22304/1/Improved%20mannan.pdf
http://psasir.upm.edu.my/id/eprint/22304/
_version_ 1643827789122502656
score 13.211869

Similar Items