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Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.

We visually identify pork adulteration in beef and chicken meatball preparations using 20 nm gold nanoparticles (GNPs) as colorimetric sensors. Meatball is a popular food in certain Asian and European countries. Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher...

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Main Authors: Ali, Md. Eaqub, Hashim, Uda, Mustafa, Shuhaimi, Che Man, Yaakob, Islam, Kh. Nurul
Format: Article
Language:English
English
Published: Hindawi Publishing Corporation 2012
Online Access:http://psasir.upm.edu.my/id/eprint/22235/1/Gold%20nanoparticle%20sensor%20for%20the%20visual%20detection%20of%20pork%20adulteration%20in%20meatball%20formulation.pdf
http://psasir.upm.edu.my/id/eprint/22235/
http://www.hindawi.com/journals/jnm/2012/103607/
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spelling my.upm.eprints.222352015-09-28T07:54:28Z http://psasir.upm.edu.my/id/eprint/22235/ Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation. Ali, Md. Eaqub Hashim, Uda Mustafa, Shuhaimi Che Man, Yaakob Islam, Kh. Nurul We visually identify pork adulteration in beef and chicken meatball preparations using 20 nm gold nanoparticles (GNPs) as colorimetric sensors. Meatball is a popular food in certain Asian and European countries. Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher food standards. Twenty nm GNPs change color from pinkish-red to gray-purple, and their absorption peak at 525 nm is red-shifted by 30–50 nm in 3 mM phosphate buffer saline (PBS). Adsorption of single-stranded DNA protects the particles against salt-induced aggregation. Mixing and annealing of a 25-nucleotide (nt) single-stranded (ss) DNA probe with denatured DNA of different meatballs differentiated well between perfectly matched and mismatch hybridization at a critical annealing temperature. The probes become available in nonpork DNA containing vials due to mismatches and interact with GNPs to protect them from salt-induced aggregation. Whereas, all the pork containing vials, either in pure and mixed forms, consumed the probes totally by perfect hybridization and turned into grey, indicating aggregation. This is clearly reflected by a well-defined red-shift of the absorption peak and significantly increased absorbance in 550–800 nm regimes. This label-free low-cost assay should find applications in food analysis, genetic screening, and homology studies. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22235/1/Gold%20nanoparticle%20sensor%20for%20the%20visual%20detection%20of%20pork%20adulteration%20in%20meatball%20formulation.pdf Ali, Md. Eaqub and Hashim, Uda and Mustafa, Shuhaimi and Che Man, Yaakob and Islam, Kh. Nurul (2012) Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation. Journal of Nanomaterials, 2012 (103607). pp. 1-7. ISSN 1687-4110; ESSN: 1687-4129 http://www.hindawi.com/journals/jnm/2012/103607/ 10.1155/2012/103607 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description We visually identify pork adulteration in beef and chicken meatball preparations using 20 nm gold nanoparticles (GNPs) as colorimetric sensors. Meatball is a popular food in certain Asian and European countries. Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher food standards. Twenty nm GNPs change color from pinkish-red to gray-purple, and their absorption peak at 525 nm is red-shifted by 30–50 nm in 3 mM phosphate buffer saline (PBS). Adsorption of single-stranded DNA protects the particles against salt-induced aggregation. Mixing and annealing of a 25-nucleotide (nt) single-stranded (ss) DNA probe with denatured DNA of different meatballs differentiated well between perfectly matched and mismatch hybridization at a critical annealing temperature. The probes become available in nonpork DNA containing vials due to mismatches and interact with GNPs to protect them from salt-induced aggregation. Whereas, all the pork containing vials, either in pure and mixed forms, consumed the probes totally by perfect hybridization and turned into grey, indicating aggregation. This is clearly reflected by a well-defined red-shift of the absorption peak and significantly increased absorbance in 550–800 nm regimes. This label-free low-cost assay should find applications in food analysis, genetic screening, and homology studies.
format Article
author Ali, Md. Eaqub
Hashim, Uda
Mustafa, Shuhaimi
Che Man, Yaakob
Islam, Kh. Nurul
spellingShingle Ali, Md. Eaqub
Hashim, Uda
Mustafa, Shuhaimi
Che Man, Yaakob
Islam, Kh. Nurul
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
author_facet Ali, Md. Eaqub
Hashim, Uda
Mustafa, Shuhaimi
Che Man, Yaakob
Islam, Kh. Nurul
author_sort Ali, Md. Eaqub
title Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
title_short Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
title_full Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
title_fullStr Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
title_full_unstemmed Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
title_sort gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation.
publisher Hindawi Publishing Corporation
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/22235/1/Gold%20nanoparticle%20sensor%20for%20the%20visual%20detection%20of%20pork%20adulteration%20in%20meatball%20formulation.pdf
http://psasir.upm.edu.my/id/eprint/22235/
http://www.hindawi.com/journals/jnm/2012/103607/
_version_ 1643827768587190272
score 13.211869

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