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Characteristics of Progesterone Synthesis In Isolated Luteal Cells

Single cells were prepared from the luteinized rat ovary, and the characteristics of progesterone synthesis by these cells were examined. The isolated luteal cells were viable and functional, and respond to luteinizing hormone (LH) stimulation by synthesizing progesterone in vitro. In the absence...

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Main Author: Tan, Cheong Huat
Format: Article
Language:English
English
Published: 1982
Online Access:http://psasir.upm.edu.my/id/eprint/2115/1/Characteristics_of_Progesterone_Synthesis_In_Isolated_Luteal_Cells.pdf
http://psasir.upm.edu.my/id/eprint/2115/
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spelling my.upm.eprints.21152013-05-27T06:58:55Z http://psasir.upm.edu.my/id/eprint/2115/ Characteristics of Progesterone Synthesis In Isolated Luteal Cells Tan, Cheong Huat Single cells were prepared from the luteinized rat ovary, and the characteristics of progesterone synthesis by these cells were examined. The isolated luteal cells were viable and functional, and respond to luteinizing hormone (LH) stimulation by synthesizing progesterone in vitro. In the absence of exogenous glucose, the rate of progesterone synthesis was 0.23 ng/ml/min. LH increased this rate 2-fold, and the duration of the initial linear phase of this reaction was sustained for up to 60 min, compared to the controls which were linear for only 15 min. This effect was dose-related: maximal stimulation was achieved with 1 J1g LH/ml, while a minimal but significant stimulation was elicited with 61 pg/ml. This steroidogenic activity was also increased by the addition of exogenous glucose and bovine serum albumin. These data serve to establish the optimum conditions for the use of single cells in studies pertaining to mechanism of LH action. 1982 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2115/1/Characteristics_of_Progesterone_Synthesis_In_Isolated_Luteal_Cells.pdf Tan, Cheong Huat (1982) Characteristics of Progesterone Synthesis In Isolated Luteal Cells. Pertanika, 5 (2). pp. 224-228. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Single cells were prepared from the luteinized rat ovary, and the characteristics of progesterone synthesis by these cells were examined. The isolated luteal cells were viable and functional, and respond to luteinizing hormone (LH) stimulation by synthesizing progesterone in vitro. In the absence of exogenous glucose, the rate of progesterone synthesis was 0.23 ng/ml/min. LH increased this rate 2-fold, and the duration of the initial linear phase of this reaction was sustained for up to 60 min, compared to the controls which were linear for only 15 min. This effect was dose-related: maximal stimulation was achieved with 1 J1g LH/ml, while a minimal but significant stimulation was elicited with 61 pg/ml. This steroidogenic activity was also increased by the addition of exogenous glucose and bovine serum albumin. These data serve to establish the optimum conditions for the use of single cells in studies pertaining to mechanism of LH action.
format Article
author Tan, Cheong Huat
spellingShingle Tan, Cheong Huat
Characteristics of Progesterone Synthesis In Isolated Luteal Cells
author_facet Tan, Cheong Huat
author_sort Tan, Cheong Huat
title Characteristics of Progesterone Synthesis In Isolated Luteal Cells
title_short Characteristics of Progesterone Synthesis In Isolated Luteal Cells
title_full Characteristics of Progesterone Synthesis In Isolated Luteal Cells
title_fullStr Characteristics of Progesterone Synthesis In Isolated Luteal Cells
title_full_unstemmed Characteristics of Progesterone Synthesis In Isolated Luteal Cells
title_sort characteristics of progesterone synthesis in isolated luteal cells
publishDate 1982
url http://psasir.upm.edu.my/id/eprint/2115/1/Characteristics_of_Progesterone_Synthesis_In_Isolated_Luteal_Cells.pdf
http://psasir.upm.edu.my/id/eprint/2115/
_version_ 1643822222555480064
score 13.211869

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