Characteristics of Progesterone Synthesis In Isolated Luteal Cells
Single cells were prepared from the luteinized rat ovary, and the characteristics of progesterone synthesis by these cells were examined. The isolated luteal cells were viable and functional, and respond to luteinizing hormone (LH) stimulation by synthesizing progesterone in vitro. In the absence...
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| Format: | Article |
| Language: | English English |
| Published: |
1982
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2115/1/Characteristics_of_Progesterone_Synthesis_In_Isolated_Luteal_Cells.pdf http://psasir.upm.edu.my/id/eprint/2115/ |
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| Summary: | Single cells were prepared from the luteinized rat ovary, and the characteristics of progesterone synthesis
by these cells were examined. The isolated luteal cells were viable and functional, and respond to
luteinizing hormone (LH) stimulation by synthesizing progesterone in vitro. In the absence of exogenous
glucose, the rate of progesterone synthesis was 0.23 ng/ml/min. LH increased this rate 2-fold, and the duration
of the initial linear phase of this reaction was sustained for up to 60 min, compared to the controls
which were linear for only 15 min. This effect was dose-related: maximal stimulation was achieved with
1 J1g LH/ml, while a minimal but significant stimulation was elicited with 61 pg/ml. This steroidogenic
activity was also increased by the addition of exogenous glucose and bovine serum albumin. These data
serve to establish the optimum conditions for the use of single cells in studies pertaining to mechanism
of LH action. |
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