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Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.

The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activi...

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Main Authors:	Md Salleh, Madihah, Liew, Shiau Tsuey, Ariff, Arbakariya
Format:	Article
Language:	English English
Published:	The Korean Society for Biotechnology and Bioengineering. 2008
Online Access:	http://psasir.upm.edu.my/id/eprint/16365/1/Profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20C.pdf http://psasir.upm.edu.my/id/eprint/16365/
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spelling	my.upm.eprints.163652015-10-26T00:30:51Z http://psasir.upm.edu.my/id/eprint/16365/ Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity(1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the solventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C.saccharobutylicum. © KSBB The Korean Society for Biotechnology and Bioengineering. 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16365/1/Profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20C.pdf Md Salleh, Madihah and Liew, Shiau Tsuey and Ariff, Arbakariya (2008) Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. Biotechnology and Bioprocess Engineering, 13 (1). pp. 33-39. ISSN 1976-3816 DOI/10.1007/s12257-007-0153-2 English
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English English
description	The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity(1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the solventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C.saccharobutylicum. © KSBB
format	Article
author	Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya
spellingShingle	Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
author_facet	Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya
author_sort	Md Salleh, Madihah
title	Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
title_short	Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
title_full	Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
title_fullStr	Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
title_full_unstemmed	Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
title_sort	profile of enzymes relevant to solvent production during direct fermentation of sago starch by c. saccharobutylicum at different ph control strategies.
publisher	The Korean Society for Biotechnology and Bioengineering.
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/16365/1/Profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20C.pdf http://psasir.upm.edu.my/id/eprint/16365/
_version_	1643826192577462272
score	13.211869

Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.

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