Formulation of protective agents for improvement of lactobacillus salivarius I 24 survival rate subjected to freese drying for production of live cells in powdered form
The effectiveness of formulations using different protective agents to maintain viability of Lactobacillus salivarius I 24 during freeze drying for production of live cell in powdered form was investigated. The influence of prefreezing and cultivation conditions on viability of cells after freez...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Science + Business Media
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14808/1/Formulation%20of%20protective%20agents%20for%20improvement%20of%20lactobacillus%20salivarius%20I%2024%20survival%20rate%20subjected%20to%20freese%20drying%20for%20production%20of%20live%20cells%20in%20powdered%20form.pdf http://psasir.upm.edu.my/id/eprint/14808/ |
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| Summary: | The effectiveness of formulations using different protective agents to maintain viability of Lactobacillus
salivarius I 24 during freeze drying for production of live
cell in powdered form was investigated. The influence of
prefreezing and cultivation conditions on viability of cells
after freeze drying was also studied. Surface methodology
was used to determine the most suitable combination of the
protective agents. Concentrations of skim milk, sucrose,
glycerol, and calcium carbonate were selected as operating
variables, and survivals of cultures after freeze drying were used as results. Skim milk and sucrose were better
protective agents than glycerol and calcium carbonate when
used individually for preserving L. salivarius I 24 during
freeze drying. Their protective abilities could be enhanced
significantly when using them as a mixture (9.85% w/v skim
milk and 10.65% w/v sucrose). Prefreezing of the cells at
−80°C for 5 h prior to freeze drying and cultivation with
regulated pH and temperature gave the highest cell viability. |
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