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Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats

In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Obdb) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19%...

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Main Authors: Mhd Jalil, Abbe Maleyki, Ismail, Amin, Chong, Pei Pei, Hamid, Muhajir, Syed Kamaruddin, Syed Hasbullah
Format: Article
Language:English
English
Published: American Chemical Society 2008
Online Access:http://psasir.upm.edu.my/id/eprint/14351/1/Effects%20of%20cocoa%20extract%20on%20glucometabolism.pdf
http://psasir.upm.edu.my/id/eprint/14351/
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spelling my.upm.eprints.143512015-10-23T08:09:55Z http://psasir.upm.edu.my/id/eprint/14351/ Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats Mhd Jalil, Abbe Maleyki Ismail, Amin Chong, Pei Pei Hamid, Muhajir Syed Kamaruddin, Syed Hasbullah In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Obdb) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight)streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight/day) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose and insulin level after 4 weeks of cocoa extract administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. Plasma free fatty acid and oxidative stress biomarker(8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in nonsupplemented rats. However, no change was observed in catalase activity. The results showed that cocoa supplementation had an effect on postprandial glucose control but not for long term (4 weeks). Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8-isoprostane and may enhance the antioxidant defense system. American Chemical Society 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14351/1/Effects%20of%20cocoa%20extract%20on%20glucometabolism.pdf Mhd Jalil, Abbe Maleyki and Ismail, Amin and Chong, Pei Pei and Hamid, Muhajir and Syed Kamaruddin, Syed Hasbullah (2008) Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats. Journal of Agricultural and Food Chemistry, 56 (17). pp. 7877-7884. ISSN 0021-8561 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Obdb) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight)streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight/day) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose and insulin level after 4 weeks of cocoa extract administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. Plasma free fatty acid and oxidative stress biomarker(8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in nonsupplemented rats. However, no change was observed in catalase activity. The results showed that cocoa supplementation had an effect on postprandial glucose control but not for long term (4 weeks). Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8-isoprostane and may enhance the antioxidant defense system.
format Article
author Mhd Jalil, Abbe Maleyki
Ismail, Amin
Chong, Pei Pei
Hamid, Muhajir
Syed Kamaruddin, Syed Hasbullah
spellingShingle Mhd Jalil, Abbe Maleyki
Ismail, Amin
Chong, Pei Pei
Hamid, Muhajir
Syed Kamaruddin, Syed Hasbullah
Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
author_facet Mhd Jalil, Abbe Maleyki
Ismail, Amin
Chong, Pei Pei
Hamid, Muhajir
Syed Kamaruddin, Syed Hasbullah
author_sort Mhd Jalil, Abbe Maleyki
title Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
title_short Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
title_full Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
title_fullStr Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
title_full_unstemmed Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
title_sort effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (ob-db) rats
publisher American Chemical Society
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/14351/1/Effects%20of%20cocoa%20extract%20on%20glucometabolism.pdf
http://psasir.upm.edu.my/id/eprint/14351/
_version_ 1643825605797478400
score 13.211869

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